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Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer
https://obihiro.repo.nii.ac.jp/records/703
https://obihiro.repo.nii.ac.jp/records/70313b49218-4f36-4335-9b9c-7bc676222d27
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
production.pdf (1.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-02-03 | |||||
| タイトル | ||||||
| タイトル | Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Interspecies | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Somatic cell nuclear transfer | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Whale | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Bhuiyan, Mohammad Musharraf Uddin
× Bhuiyan, Mohammad Musharraf Uddin× Suzuki, Yo× Watanabe, Hiroyuki× Lee, Eunsong× Hirayama, Hiroki× Matsuoka, Koji× Fujise, Yoshihiro× Ishikawa, Hajime× Ohsumi, Seiji× Fukui, Yutaka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale （Balaenoptera borealis) interspecies somatic cell nuclear transfer （iSCNT) embryos. Moreover, trichostatin A （TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher （88.1%) in embryos reconstructed using the intracytoplasmic cell injection method （ICI) than that （48.7%) in the subzonal cell insertion （SUZI) counterpart. The rates of pseudopronucleus （PPN) formation （77.4 vs. 77.2%) and cleavage （24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were significantly （P<0.05) lower in the iSCNT embryos than in the parthenogenetic control （95.7% and 64.4%, respectively). Although 21.5% of the bovine parthenogenetic embryos developed to the blastocyst stage, no iSCNT embryo developed beyond the 6-cell stage. In Experiment 2, the cleavage rate did not vary between the TSA （50 nM)-treated and non-treated whale iSCNT embryos （30.5 vs. 32.3%, respectively). Moreover, it did not vary between the TSA-treated iSCNT and SCNT embryos （30.5 vs. 32.0%, respectively). Only one TSA non-treated iSCNT embryo developed to a compacted morula with 20 nuclei. One TSA-treated whale SCNT embryo developed to the 8-cell stage, and out of five whale iSCNT embryos, a 6-cell stage embryo was positive for whale DNA. In conclusion, bovine oocytes have the ability to support development of sei whale nuclei up to the 6-cell stage. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of Reproduction and Development 巻 56, 号 1, p. 131-139, 発行日 2010 |
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| 出版者 | ||||||
| 出版者 | Society for Reproduction and Development | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 09168818 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10936678 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1262/jrd.09-158A | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Japanese Society of Animal Reproduction | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
