{"created":"2023-05-15T15:15:10.223549+00:00","id":703,"links":{},"metadata":{"_buckets":{"deposit":"97f21232-74c2-4223-a3df-f1bc31045bd3"},"_deposit":{"created_by":12,"id":"703","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"703"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000703","sets":["5"]},"author_link":["1517","1520","1515","350","1519","572","1521","1523","1522","344"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"139","bibliographicPageStart":"131","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Journal of Reproduction and Development","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale （Balaenoptera borealis) interspecies somatic cell nuclear transfer （iSCNT) embryos. Moreover, trichostatin A （TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher （88.1%) in embryos reconstructed using the intracytoplasmic cell injection method （ICI) than that （48.7%) in the subzonal cell insertion （SUZI) counterpart. The rates of pseudopronucleus （PPN) formation （77.4 vs. 77.2%) and cleavage （24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were significantly （P<0.05) lower in the iSCNT embryos than in the parthenogenetic control （95.7% and 64.4%, respectively). Although 21.5% of the bovine parthenogenetic embryos developed to the blastocyst stage, no iSCNT embryo developed beyond the 6-cell stage. In Experiment 2, the cleavage rate did not vary between the TSA （50 nM)-treated and non-treated whale iSCNT embryos （30.5 vs. 32.3%, respectively). Moreover, it did not vary between the TSA-treated iSCNT and SCNT embryos （30.5 vs. 32.0%, respectively). Only one TSA non-treated iSCNT embryo developed to a compacted morula with 20 nuclei. One TSA-treated whale SCNT embryo developed to the 8-cell stage, and out of five whale iSCNT embryos, a 6-cell stage embryo was positive for whale DNA. In conclusion, bovine oocytes have the ability to support development of sei whale nuclei up to the 6-cell stage.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Reproduction and Development","subitem_publisher_language":"en"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1262/jrd.09-158A","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Japanese Society of Animal Reproduction","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10936678","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09168818","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Bhuiyan, Mohammad Musharraf Uddin","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Yo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lee, Eunsong","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirayama, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuoka, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujise, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Ishikawa, Hajime","creatorNameLang":"en"}],"familyNames":[{}],"givenNames":[{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ohsumi, Seiji","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Fukui, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"production.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"production.pdf","url":"https://obihiro.repo.nii.ac.jp/record/703/files/production.pdf"},"version_id":"047d46d6-9397-437c-bdc6-0cd1194bba91"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Interspecies","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Somatic cell nuclear transfer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Whale","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-02-03"},"publish_date":"2011-02-03","publish_status":"0","recid":"703","relation_version_is_last":true,"title":["Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-04-23T01:12:20.059685+00:00"}