@article{oai:obihiro.repo.nii.ac.jp:00000703,
 author = {Bhuiyan, Mohammad Musharraf Uddin and Suzuki, Yo and Watanabe, Hiroyuki and Lee, Eunsong and Hirayama, Hiroki and Matsuoka, Koji and Fujise, Yoshihiro and Ishikawa, Hajime and Ohsumi, Seiji and Fukui, Yutaka},
 issue = {1},
 journal = {Journal of Reproduction and Development},
 month = {},
 note = {application/pdf, The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale （Balaenoptera borealis) interspecies somatic cell nuclear transfer （iSCNT) embryos. Moreover, trichostatin A （TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher （88.1%) in embryos reconstructed using the intracytoplasmic cell injection method （ICI) than that （48.7%) in the subzonal cell insertion （SUZI) counterpart. The rates of pseudopronucleus （PPN) formation （77.4 vs. 77.2%) and cleavage （24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were significantly （P<0.05) lower in the iSCNT embryos than in the parthenogenetic control （95.7% and 64.4%, respectively). Although 21.5% of the bovine parthenogenetic embryos developed to the blastocyst stage, no iSCNT embryo developed beyond the 6-cell stage. In Experiment 2, the cleavage rate did not vary between the TSA （50 nM)-treated and non-treated whale iSCNT embryos （30.5 vs. 32.3%, respectively). Moreover, it did not vary between the TSA-treated iSCNT and SCNT embryos （30.5 vs. 32.0%, respectively). Only one TSA non-treated iSCNT embryo developed to a compacted morula with 20 nuclei. One TSA-treated whale SCNT embryo developed to the 8-cell stage, and out of five whale iSCNT embryos, a 6-cell stage embryo was positive for whale DNA. In conclusion, bovine oocytes have the ability to support development of sei whale nuclei up to the 6-cell stage.},
 pages = {131--139},
 title = {Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer},
 volume = {56},
 year = {2010}
}