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Neospora GRA6 possesses immune-stimulating activity and confers efficient protection against Neospora caninum infection in mice
https://obihiro.repo.nii.ac.jp/records/4345
https://obihiro.repo.nii.ac.jp/records/43454b68cf9f-0f15-425b-ae99-377d11578035
| 名前 / ファイル | ライセンス | アクション |
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2019-04-09 | |||||||||
| タイトル | ||||||||||
| タイトル | Neospora GRA6 possesses immune-stimulating activity and confers efficient protection against Neospora caninum infection in mice | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Immunity | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | NcGRA6 | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Neospora caninum | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Neosporosis | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Vaccination | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Vaccine | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Fereig, Ragab M.
× Fereig, Ragab M.× Shimoda, Naomi× Abdelbaky, Hanan H.× Kuroda, Yasuhiro× 西川, 義文
WEKO
176
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Vaccination has the potential to be the most cost-effective control measure for reducing the economic burden of neosporosis in cattle. In this study, the immune-stimulatory effect of recombinant Neospora caninum dense granule protein 6 (NcGRA6) was confirmed via its triggering of IL-12p40 production in murine macrophages. BALB/c mice were immunized with recombinant NcGRA6 fused with glutathione S-transferase (GST) protein with or without oligomannose-coated-liposomes (OMLs) as the potential adjuvant. Specific IgG1 antibody production was observed from 21 and 35 days after the first immunization in NcGRA6+GST- and NcGRA6+GST-OML-immunized mice, respectively. However, specific IgG2a was detected 1 week after the infection, and IgG2a levels of the NcGRA6+GST- group were higher than those of the NcGRA6+GST-OML-group. Moreover, spleen cell proliferation with concomitant interferon-gamma production was detected in mice immunized with NcGRA6+GST, indicating that a significant cellular immune response was induced. Mouse survival rates against N. caninum challenge infection were 91.7% for NcGRA6+GST and 83.3% for NcGRA6+GST-OML, which were significantly higher than those of control groups (GST-OML: 25%, phosphate-buffered saline: 16.7%). This indicates that naked NcGRA6+GST induced protective immunity. Thus, our findings highlight the immune-stimulating potential of NcGRA6 and the ability to induce protective immunity against N. caninum infection in mice. © 2019 Elsevier B.V. | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
en : Veterinary Parasitology 巻 267, p. 61-68, 発行日 2019-03 |
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| 出版者 | ||||||||||
| 出版者 | Elsevier | |||||||||
| 言語 | en | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03044017 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA00883655 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | info:doi/10.1016/j.vetpar.2019.02.003 | |||||||||
| 権利 | ||||||||||
| 言語 | en | |||||||||
| 権利情報 | This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.2/> | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 日本十進分類法 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 649 | |||||||||
