| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-01-16 |
| タイトル |
|
|
タイトル |
Development of CpGP15 recombinant antigen of Cryptosporidium parvum for detection of the specific antibodies in cattle |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cattle |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CpGP15 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CpP23 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cryptosporidium parvum |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ELISA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Japan |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ichikawa-Seki, Madoka
Fereig, Ragab M.
Masatani, Tatsunori
Kinami, Aiko
Takahashi, Yoko
木田, 克弥
西川, 義文
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The infection of neonatal calves with Cryptosporidium parvum can have a huge economic impact because diarrhea caused by the parasite sometimes results in death. A serodiagnostic system will be helpful in the diagnosis of C. parvum infection. CpP23 is commonly used as an antigen for enzyme-linked immunosorbent assay (ELISA); however, some positive sera show low reactivities, as shown in this study. Herein, we focused on three other antigens, CpGP15, CpP2 and CpGP60, in addition to CpP23, to detect C. parvum-specific antibodies in cattle sera. CpP23 and CpGP15 showed substantial ability to discriminate between positive (n = 10) and negative (n = 10) control cattle sera. Unlike our previous report, both the sensitivity and the specificity were 100% when the two antigens were employed for the ELISA. The newly developed ELISA was applied to a total of 344 sera obtained from 9 cattle farms. Two farms among them had suffered from C. parvum infections before, and were regarded as the C. parvum-positive farms. The positive rates of antibodies against CpP23 and CpGP15 in the C. parvum-positive farms were 42.7% and 49.8%, respectively, whereas the positive rate for either of the antigens was 63.0% in the farms. In contrast, 14.3% and 9.8% were positive for CpP23 and CpGP15 in the C. parvum-negative farms, respectively, whereas 18.8% was positive for either of the antigens. This study revealed that the ELISAs employing both of CpP23 and CpGP15 can avoid false-negative results and are useful for monitoring of the C. parvum infection in cattle farms. © 2018 Elsevier B.V. |
|
言語 |
en |
| 書誌情報 |
en : Parasitology International
巻 69,
p. 8-12,
発行日 2019-04
|
| 出版者 |
|
|
出版者 |
Elsevier Ireland |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
13835769 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11112001 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1016/j.parint.2018.10.013 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.5/> |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
649 |
full text (3.6 MB)
