{"created":"2023-05-15T15:18:01.770777+00:00","id":4302,"links":{},"metadata":{"_buckets":{"deposit":"cbe81afb-eeb2-461b-92ac-928fc95198ee"},"_deposit":{"created_by":12,"id":"4302","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"4302"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00004302","sets":["5"]},"author_link":["348","6128","2160","93","6127","378","176"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-04","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"12","bibliographicPageStart":"8","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"Parasitology International","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The infection of neonatal calves with Cryptosporidium parvum can have a huge economic impact because diarrhea caused by the parasite sometimes results in death. A serodiagnostic system will be helpful in the diagnosis of C. parvum infection. CpP23 is commonly used as an antigen for enzyme-linked immunosorbent assay (ELISA); however, some positive sera show low reactivities, as shown in this study. Herein, we focused on three other antigens, CpGP15, CpP2 and CpGP60, in addition to CpP23, to detect C. parvum-specific antibodies in cattle sera. CpP23 and CpGP15 showed substantial ability to discriminate between positive (n = 10) and negative (n = 10) control cattle sera. Unlike our previous report, both the sensitivity and the specificity were 100% when the two antigens were employed for the ELISA. The newly developed ELISA was applied to a total of 344 sera obtained from 9 cattle farms. Two farms among them had suffered from C. parvum infections before, and were regarded as the C. parvum-positive farms. The positive rates of antibodies against CpP23 and CpGP15 in the C. parvum-positive farms were 42.7% and 49.8%, respectively, whereas the positive rate for either of the antigens was 63.0% in the farms. In contrast, 14.3% and 9.8% were positive for CpP23 and CpGP15 in the C. parvum-negative farms, respectively, whereas 18.8% was positive for either of the antigens. This study revealed that the ELISAs employing both of CpP23 and CpGP15 can avoid false-negative results and are useful for monitoring of the C. parvum infection in cattle farms. © 2018 Elsevier B.V.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Ireland","subitem_publisher_language":"en"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.parint.2018.10.013","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.5/>","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11112001","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13835769","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ichikawa-Seki, Madoka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2160","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fereig, Ragab M.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"378","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000365525697","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000365525697"}]},{"creatorNames":[{"creatorName":"Masatani, Tatsunori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"348","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000018775604","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000018775604"},{"nameIdentifier":"70614072","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70614072"},{"nameIdentifier":"tmasatani","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/tmasatani"}]},{"creatorNames":[{"creatorName":"Kinami, Aiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6127","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Yoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6128","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Kida, Katsuya","creatorNameLang":"en"},{"creatorName":"木田, 克弥","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Kida","familyNameLang":"en"},{"familyName":"木田","familyNameLang":"ja"}],"givenNames":[{"givenName":"Katsuya","givenNameLang":"en"},{"givenName":"克弥","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"93","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014478828","nameIdentifierScheme":"CiNii 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ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000000186605"},{"nameIdentifier":"90431395","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90431395"},{"nameIdentifier":"read0136808","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0136808"},{"nameIdentifier":"0000-0001-5005-6377","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-5005-6377"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-01"}],"displaytype":"detail","filename":"manuscript.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"full text","url":"https://obihiro.repo.nii.ac.jp/record/4302/files/manuscript.pdf"},"version_id":"d07851a5-74ad-4795-b451-0cb8b8e3bc15"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cattle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CpGP15","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CpP23","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryptosporidium parvum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Japan","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of CpGP15 recombinant antigen of Cryptosporidium parvum for detection of the specific antibodies in cattle","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of CpGP15 recombinant antigen of Cryptosporidium parvum for detection of the specific antibodies in cattle","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-01-16"},"publish_date":"2019-01-16","publish_status":"0","recid":"4302","relation_version_is_last":true,"title":["Development of CpGP15 recombinant antigen of Cryptosporidium parvum for detection of the specific antibodies in cattle"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-12-04T02:18:47.540528+00:00"}