WEKO3
アイテム
In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers
https://obihiro.repo.nii.ac.jp/records/1726
https://obihiro.repo.nii.ac.jp/records/1726bc6da5e3-a503-4714-9f53-6f0099c33d60
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Prot.Vol.1-(1).pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-12-21 | |||||
| タイトル | ||||||
| タイトル | In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | feeder cell layers | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | in vitro cultivation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | trypomastigotes | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Hirumi, Hiroyuki
× Hirumi, Hiroyuki× Hirumi, K.× Moloo, S. K.× Shaw, M. K. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II: metacyclic-producing cultures of T. vivax IL 3671 were initiated with BSFs derived from infected cattle, without feeder layers at / 27 OC using HMI-107 medium. Metacyclics were then transferred to the axenic culture conditions established in System I. They also transformed to BSFs and multiplied in HMI-162 and HMI-163 media. System III: cultures were initiated with proboscides of Glossina morsitans centralis which were infected with T. vivax IL 3671, without feeder layers at 34 OC using HMI-162 medium. Metacyclics emerged from the proboscides, transformed to BSFs and continued to grow in HMI-163 medium. The maximum density and the population doubling time of IL 3671 BSFs in HMI-163 medium were 1.8 x 106 / ml and 16.1 h, respectively. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : The journal of protozoology research 巻 1, p. 1-12, 発行日 1991-10 |
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| 出版者 | ||||||
| 出版者 | Research Center for Protozoan Molecular Immunology | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 09174427 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10824856 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Research Center for Protozoan Molecular Immunology | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 言語 | en | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 649 | |||||
