{"created":"2023-05-15T15:15:59.383128+00:00","id":1726,"links":{},"metadata":{"_buckets":{"deposit":"7cd27acb-f3f4-4f57-b312-598faba63232"},"_deposit":{"created_by":12,"id":"1726","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1726"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00001726","sets":["12:73"]},"author_link":["343","3995","3996","3997"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"12","bibliographicPageStart":"1","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"The journal of protozoology research","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II: metacyclic-producing cultures of T. vivax IL 3671 were initiated with BSFs derived from infected cattle, without feeder layers at / 27 OC using HMI-107 medium. Metacyclics were then transferred to the axenic culture conditions established in System I. They also transformed to BSFs and multiplied in HMI-162 and HMI-163 media. System III: cultures were initiated with proboscides of Glossina morsitans centralis which were infected with T. vivax IL 3671, without feeder layers at 34 OC using HMI-162 medium. Metacyclics emerged from the proboscides, transformed to BSFs and continued to grow in HMI-163 medium. The maximum density and the population doubling time of IL 3671 BSFs in HMI-163 medium were 1.8 x 106 / ml and 16.1 h, respectively.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Research Center for Protozoan Molecular Immunology","subitem_publisher_language":"en"}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Research Center for Protozoan Molecular Immunology","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824856","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09174427","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirumi, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"343","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000001143733","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000001143733"}]},{"creatorNames":[{"creatorName":"Hirumi, K.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3995","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Moloo, S. K.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3996","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shaw, M. K.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3997","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"Prot.Vol.1-(1).pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Prot.Vol.1-(1).pdf","url":"https://obihiro.repo.nii.ac.jp/record/1726/files/Prot.Vol.1-(1).pdf"},"version_id":"dc6d776e-6dea-46a0-b8a3-211fbdce2b56"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"feeder cell layers","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro cultivation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"trypomastigotes","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["73"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-12-21"},"publish_date":"2006-12-21","publish_status":"0","recid":"1726","relation_version_is_last":true,"title":["In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2023-10-26T07:52:39.117774+00:00"}