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Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
N090202-1.pdf (267.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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| 公開日 | 2009-02-02 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Babesia gibsoni | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | enzyme-linked immunosorbent assay | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | deiagnostic performance | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 著者 |
Terkawi, M. A.
× Terkawi, M. A.
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359
× Aboge, G.× Jia, H.× Goo, Y-K.× Ooka, H.× 山岸, 潤也
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330
× 西川, 義文
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176
× 河津, 信一郎× Fujisaki, K.× 玄, 学南
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295
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| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both extracellular and intracellular merozoite. Furthermore, recombinant BgP27 was used as an antigen in an enzyme-linked immunosorbent assay (ELISA). Thus, the kinetics of the anti-BgP27 antibody was detected in serial serum samples collected from a B. gibsoni-infected dog. IgG levels were high throughout the course of infection. In addition, the ELISA was able to differentiate between B. gibsoni-infected dog serum and B. canis subspecies-infected dog serum or normal dog serum. The diagnostic performance of BgP27-ELISA revealed the potential use of the antigen for detection of infection in dogs. | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 書誌情報 |
en : The journal of protozoology research 巻 18, 号 2, p. 48-56, 発行日 2008-12 |
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| 出版者 | ||||||||||||||||||||
| 出版者 | National Research Center for Protozoan Diseases | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||||
| 収録物識別子 | 09174427 | |||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA10824856 | |||||||||||||||||||
| 権利 | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 権利情報 | National Research Center for Protozoan Diseases | |||||||||||||||||||
| フォーマット | ||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| 日本十進分類法 | ||||||||||||||||||||
| 主題Scheme | NDC | |||||||||||||||||||
| 主題 | 649 | |||||||||||||||||||
