{"created":"2023-05-15T15:15:47.666181+00:00","id":1467,"links":{},"metadata":{"_buckets":{"deposit":"f5b53c33-4583-4117-8144-6bed01bc8264"},"_deposit":{"created_by":12,"id":"1467","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1467"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00001467","sets":["12:21"]},"author_link":["341","330","3107","340","295","359","3105","3106","176","90"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"56","bibliographicPageStart":"48","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"The journal of protozoology research","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both extracellular and intracellular merozoite. Furthermore, recombinant BgP27 was used as an antigen in an enzyme-linked immunosorbent assay (ELISA). Thus, the kinetics of the anti-BgP27 antibody was detected in serial serum samples collected from a B. gibsoni-infected dog. IgG levels were high throughout the course of infection. In addition, the ELISA was able to differentiate between B. gibsoni-infected dog serum and B. canis subspecies-infected dog serum or normal dog serum. The diagnostic performance of BgP27-ELISA revealed the potential use of the antigen for detection of infection in dogs.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Research Center for Protozoan Diseases","subitem_publisher_language":"en"}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"National Research Center for Protozoan Diseases","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824856","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09174427","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Terkawi, M. A.","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Aboge, G.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jia, H.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goo, Y-K.","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ooka, H.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamagishi, Junya","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Nishikawa, Yoshifumi","creatorNameLang":"en"},{"creatorName":"西川, 義文","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Kawazu, Shin-ichiro","creatorNameLang":"en"},{"creatorName":"河津, 信一郎","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Fujisaki, K.","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Xuan, Xuenan","creatorNameLang":"en"},{"creatorName":"玄, 学南","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"N090202-1.pdf","filesize":[{"value":"267.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"N090202-1.pdf","url":"https://obihiro.repo.nii.ac.jp/record/1467/files/N090202-1.pdf"},"version_id":"359a2b35-4fd8-4ea3-9139-a48f19ca38c5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Babesia gibsoni","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"enzyme-linked immunosorbent assay","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"deiagnostic performance","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["21"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-02-02"},"publish_date":"2009-02-02","publish_status":"0","recid":"1467","relation_version_is_last":true,"title":["Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-20T07:45:28.849180+00:00"}