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Comparison of protein profile of co-existing Fasciola hepatica and Fasciola gigantica parasite in Bos taurus (cattle) and Bubalus bubalis (Philippine water buffalo)
https://obihiro.repo.nii.ac.jp/records/1460
https://obihiro.repo.nii.ac.jp/records/146082e1ea7b-fd73-4830-a5f4-cebf19d13be6
名前 / ファイル | ライセンス | アクション |
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v19-1-9.pdf (330.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-10-01 | |||||
タイトル | ||||||
タイトル | Comparison of protein profile of co-existing Fasciola hepatica and Fasciola gigantica parasite in Bos taurus (cattle) and Bubalus bubalis (Philippine water buffalo) | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cattle & carabao Fasciola spp | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | protein profile | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | SDS-PAGE | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Philippines | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
De, Vera M.E
× De, Vera M.E× Sato, K× Oyong, G× Claveria, F.G |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host species-related influence. Between cattle and bubaline F. hepatica, six bands (220kDa, 150kDa, 115kDa, 67kDa, 34-37kDa, 30kDa) were bubaline-specific; between cattle and bubaline F. gigantica, four bands (212kDa, 150kDa, 70kDa, 30kDa) were bubaline-specific, while only three bands were shared (150kDa, 67-70kDa, 30kDa) by bubaline F. hepatica and F. gigantica. Current molecular findings represent the first in the country, where fascioliasia is prevalent. Confirmation of these results entails protein profiling of extracts of freshly-collected individual worms alongside extracts of infected and non-infected liver tissue samples, to mark out host-derived proteins. Its surveillance in susceptible host species in farms around the country, jointly with analysis of morphological and morphometric data of co-existing fasciolid species is highly recommended. | |||||
言語 | en | |||||
書誌情報 |
en : The journal of protozoology research 巻 19, 号 1, p. 1-9, 発行日 2009-06 |
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出版者 | ||||||
出版者 | National Research Center for Protozoan Diseases | |||||
言語 | en | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 09174427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824856 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | National Research Center for Protozoan Diseases | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 649 |