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Specific Molecular Detection and Characterization of Anaplasma marginale in Mongolian Cattle
https://obihiro.repo.nii.ac.jp/records/956
https://obihiro.repo.nii.ac.jp/records/9566208bd75-b926-4e9b-ab9f-194b11cfc2e1
名前 / ファイル | ライセンス | アクション |
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inokuma75.399-406.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2014-05-19 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Specific Molecular Detection and Characterization of Anaplasma marginale in Mongolian Cattle | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Anaplasma marginale | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | cattle | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Mongolia | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Msp5 | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | PCR | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Ybañez, Adrian Patalinghug
× Ybañez, Adrian Patalinghug× Sivakumar, Thillaiampalam× Battsetseg, Badgar× Battur, Banzragch× Altangerel, Khukhuu× 松本, 高太郎× 横山, 直明
WEKO
312
× 猪熊, 壽
WEKO
61
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA fragments of an Anaplasma sp. closely related to A. ovis (with 95.0% identity) were detected using a different nPCR method based on groEL gene. The phylogenetic analyses based on the Msp5, groEL and 16S rRNA genes demonstrated that A. marginale isolates in Mongolia were not divergent from the isolates distributed in other countries. The present study successfully established a new nPCR assay that can detect A. marginale, and reported the first molecular detection and characterization of A. marginale and an Anaplasma sp. closely related to A. ovis in Mongolian cattle populations. | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : Journal of Veterinary Medical Science 巻 75, 号 4, p. 399-406, 発行日 2013 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | Japanese Society of Veterinary Science | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 09167250 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA10796138 | |||||||||||||
DOI | ||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | info:doi/10.1292/jvms.12-0361 | |||||||||||||
権利 | ||||||||||||||
言語 | en | |||||||||||||
権利情報 | Japanese Society of Veterinary Science | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | VoR | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |