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アイテム
Effect of Human Milk Oligosaccharides on Messenger Ribonucleic Acid Expression of Toll-like Receptor 2 and 4, and of MD2 in the Intestinal Cell Line HT-29
https://obihiro.repo.nii.ac.jp/records/746
https://obihiro.repo.nii.ac.jp/records/746a30222e3-02a3-4924-a776-8488a7b8bbaf
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
urashima57(3),177-183,2010.pdf (443.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2015-10-30 | |||||||||
| タイトル | ||||||||||
| タイトル | Effect of Human Milk Oligosaccharides on Messenger Ribonucleic Acid Expression of Toll-like Receptor 2 and 4, and of MD2 in the Intestinal Cell Line HT-29 | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | human milk oligosaccharides | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | galacto-oligosaccharides | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | toll-like receptor | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | intestinal cell | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | HT-29 cells | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | 腸管細胞株HT-29におけるヒトミルクオリゴ糖のToll様受容体2，4およびMD2遺伝子発現に及ぼす影響 | |||||||||
| 言語 | ja | |||||||||
| 著者 |
朝隈, 貞樹
× 朝隈, 貞樹× Yokoyama, Tomoko× Kimura, Kazumasa× Watanabe, Yoko× 中村, 正× 福田, 健二× 浦島, 匡
WEKO
291
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | It is believed that human milk oligosaccharides （HMO) act as receptor analogues that inhibit the attachment of pathogenic microorganisms to the infant colon, but the direct involvement of HMO in anti infection has not as yet been demonstrated. The present study was conducted to clarify whether HMOs as well as commercially available galacto oligosaccharides （GO), from which lactose has been removed, affect the expression of toll-like receptor （TLR) 2 and 4, and of MD2 mRNA in the HT-29, human colonic cell line. HT-29 cells were treated with neutral human milk oligosaccharides （nHMOs), which had been separated from the acidic oligosaccharide fraction and also from lactose, as well as with GOs. HT-29 cells were treated with specific oligosaccharides of major components of nHMO or GO. The mRNA expression of TLR2, 4 and MD2 were measured using Real-time RT-PCR normalized to glyceraldehydes 3-phosphate dehydrogenase （GAPDH) mRNA expression. The addition of nHMO affected the expression of TLR2. Treatment with 1.0 mg/mL of nHMO as well as with 0.5 mg and 1.0 mg/mL GO enhanced the mRNA expression of TLR4. In experiments with specific oligosaccharides, treatments with 3'-sialyllactose （3'-SL), 6'-sialyllactose （6'-SL) or 6'-galactosyllactose （6'-GL) increased the expression of TLR-2, while the administration of lacto-N-fucopentaose I （LNFP I), 3'-, 6'-SL or 6'-GL enhanced that of TLR4. These results suggest that HMOs as well as GOs had a direct effect on colonic epithelial cells, with induction of the mRNA expression of TLR. | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
en : Journal of Applied Glycoscience 巻 57, 号 3, p. 177-183, 発行日 2010 |
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| 出版者 | ||||||||||
| 出版者 | Japanese Society of Applied Glycoscience | |||||||||
| 言語 | en | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 13447882 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA11809133 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | info:doi/10.5458/jag.57.177 | |||||||||
| 権利 | ||||||||||
| 言語 | en | |||||||||
| 権利情報 | Japanese Society of Applied Glycoscience | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
