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Enhanced Oocyte Activation by Intracytoplasmic Injection of Porcine Spermatozoa Pre-treated with Dithiothreitol
https://obihiro.repo.nii.ac.jp/records/627
https://obihiro.repo.nii.ac.jp/records/627545d3ac5-adfc-4dd6-b6a9-49f52a0b53df
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
enhanced.pdf (799.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-01-31 | |||||
| タイトル | ||||||
| タイトル | Enhanced Oocyte Activation by Intracytoplasmic Injection of Porcine Spermatozoa Pre-treated with Dithiothreitol | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Assisted reproductive technology | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Dithiothreitol | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Intracytoplasmic sperm injection | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Oocyte activation | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | ブタ顕微授精における精子の Dithiothreitol 処理による卵子活性化の促進 | |||||
| 言語 | ja | |||||
| 著者 |
Watanabe, Hiroyuki
× Watanabe, Hiroyuki× Akiyama, Junko× Bhuiyan, Mohammad Musharraf Uddin× Fukui, Yutaka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of this study was to confirm the effect of dithiothreitol (DTT)-treated spermatozoa on oocyte activation following intracytoplasmic sperm injection (ICSI). Boar spermatozoa with or without DTT treatment (5 mM, 30 min) were injected into in vitro matured porcine oocytes, and the nuclear phase in presumptive zygotes was observed at 3 h intervals up to 12 h after ICSI. Furthermore, developmental competence of embryos produced by DTT-treated or non-treated spermatozoa was monitored after cultivation in vitro for 144 h. Male and female pronuclear formation rates in the oocytes injected with DTT-treated spermatozoa were significantly (P < 0.05) higher than those in the oocytes injected with non-treated spermatozoa. Additionally, we observed that female pronuclear formation was linked to male pronuclear formation. Sperm treatment with DTT improved (P < 0.05) subsequent development up to the blastocyst stage. These findings confirm the efficiency of DTT in in vitro porcine embryo production mediated by ICSI. We conclude that DTT treatment improves the formation of not only male pronuclei but also female pronuclei in porcine ICSI. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 日本哺乳動物卵子学会誌 en : Journal of Mammalian Ova Research 巻 26, 号 1, p. 54-60, 発行日 2009 |
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| 出版者 | ||||||
| 出版者 | Japanese Society of Mammalian Ova Research | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 13417738 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10548943 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1274/jmor.26.54 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Japan Society for Ova Research | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
