WEKO3
アイテム
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Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells
https://obihiro.repo.nii.ac.jp/records/481
https://obihiro.repo.nii.ac.jp/records/481bfc3d47b-036f-4d37-bbc6-0f99c1f8a984
名前 / ファイル | ライセンス | アクション |
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shimizu53(1),105-112,2007.pdf (138.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2015-03-31 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Estradiol | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Fetal liver kinase (Flk-1) | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Fms-like tyrosine kinase (Flt-1) | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Follicle-stimulating hormone (FSH) | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Granulosa cell | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Vascular Endothelial Growth factor (VEGF) | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
清水, 隆
× 清水, 隆× Jayawardana, Barana C.× 手塚, 雅文
WEKO
278
× 宮本, 明夫
WEKO
151
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | Vascular endothelial growth factor (VEGF) isoforms (VEGF 120 and VEGF 164) secreted by granulosa cells are involved in thecal angiogenesis during follicular development in the bovine ovary. However, whether the transcript of the VEGF120 and VEGF164 isoforms differs during follicular development in the ovary is still unknown. We first examined the gene expression of VEGF120, VEGF164, fms-like tyrosine kinase (Flt-1), and fetal liver kinase (Flk-1) in the granulosa cells (GCs) and theca cells (TCs) of pre-selection and post-selection follicles (PRF and POF respectively) from the bovine ovary. Then we examined the effects of FSH and estradiol (E2) on these factors in cultured bovine GCs. Messenger RNA (mRNA) expression was quantified using real-time PCR methods. The concentrations of E2 and P4 in the follicular fluid (FF) of the PRF and POF were estimated using an enzyme immunoassay (EIA). The concentrations of E2 and P4 in the FF were significantly higher in the POF than in the PRF. The ratio of E2/P4 in PRF and POF was 0.37 and 3.8, respectively. The expression levels of the VEGF120, VEGF164, and Flk-1 mRNAs in the GCs of POF with high E2 concentration were higher than those of PRF. The levels of the Flt-1 and Flk-1 mRNAs in the TCs were not different between PRF and POF. Since E2 in the FF of the POF used in the present study was high compared with the PRF, we examined the effects of E2 and FSH on the expression of the above genes using cultured GCs. Expression of VEGF120 mRNA was induced by a low concentration (1 ng/ml) of E2, whereas the levels of VEGF164 and Flk-1 mRNAs were not affected by E2. FSH stimulated the expression of the VEGF isoforms and Flk-1 genes. Moreover, the expression of those genes was enhanced when low E2 (1 ng/ml) was added to FSH. In conclusion, our data indicates that the VEGF isoforms have a follicle stage-dependent expression pattern. Thus, our results suggest that the expression of VEGF isoforms may be associated with characterization of the preovulatory phenotype during follicle development in the bovine ovary. | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : Journal of Reproduction and Development 巻 53, 号 1, p. 105-112, 発行日 2007 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | Japanese Society of Animal Reproduction | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 09168818 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA10936678 | |||||||||||||
DOI | ||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | info:doi/10.1262/jrd.18088 | |||||||||||||
権利 | ||||||||||||||
言語 | en | |||||||||||||
権利情報 | Japanese Society of Animal Reproduction | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | VoR | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |