WEKO3
アイテム
Improvement of SYBR Green I−based fluorescence assay reading procedure for anti−babesial drugs screening in vitro
https://obihiro.repo.nii.ac.jp/records/4626
https://obihiro.repo.nii.ac.jp/records/4626ec9b4236-d3f8-4189-b0a8-15254c377a87
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fulltext (885.4 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2020-02-10 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Improvement of SYBR Green I−based fluorescence assay reading procedure for anti−babesial drugs screening in vitro | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Fluorescence | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Babesia | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | SYBR Green | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Freeze thaw | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
Munkhjargal, Tserendorj
× Munkhjargal, Tserendorj× Batiha, Gaber El-Saber× Beshbishy, Amany Magdy× Rizk, Mohamed A.× Guswanto, Azirwan× Onikubo, Toshi× Dickson, Tayebwa× 横山, 直明
WEKO
312
× 五十嵐, 郁男
WEKO
58
|
|||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Drug susceptibility of the Babesia parasite in vitro has been used to assess the anti-babesial activities of new candidates. Recently, the in vitro drug susceptibility assay has been performed by SYBR Green I (SG I) -based fluorescence assay. Herein, we investigated the validity of a fluorescence assay for the shipping process under various culture conditions. The highest fluorescence signal was observed in freeze-thaw of Babesia and Theileria parasites cultures incubated with lysis buffer containing SG I for a half hour. This result indicates the ability of this additional step to lysis of infected RBCs completely with releasing of parasite nucleic acid. Such that, the nucleic acid can interact with SG I stain. All tested drugs showed potential anti-babesial activity and slightly high selective index against the in vitro growth of the Babesia and Theileria parasites. The IC50 values generated using the fluorescence assay with additional freeze-thaw step is comparable with those of IC50 values obtained from the unmodified fluorescence assay and microscopy method. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : The Journal of protozoology research 巻 29, 号 1-2, p. 26-43, 発行日 2019 |
|||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | National Research Center for Protozoan Diseases | |||||||||||||
| 言語 | en | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||
| 収録物識別子 | 09174427 | |||||||||||||
| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA10824856 | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | National Research Center for Protozoan Diseases | |||||||||||||
| フォーマット | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | application/pdf | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 日本十進分類法 | ||||||||||||||
| 主題Scheme | NDC | |||||||||||||
| 主題 | 649 | |||||||||||||
