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Preliminary application and evaluation of loop-mediated isothermal amplification (LAMP) for detection of bovine theileriosis and trypanosomosis in Tanzania
https://obihiro.repo.nii.ac.jp/records/4466
https://obihiro.repo.nii.ac.jp/records/4466bf62aa02-f800-496c-80f3-0816b2852865
名前 / ファイル | ライセンス | アクション |
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fulltext (208.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2019-11-07 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Preliminary application and evaluation of loop-mediated isothermal amplification (LAMP) for detection of bovine theileriosis and trypanosomosis in Tanzania | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Bovine theileria spp. | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | LAMP | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Microscopy | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | PCR | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Tanzania Trypanosoma congolense | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Thekisoe, Oriel M. M.
× Thekisoe, Oriel M. M.× Omolo, J.D.× Swai, E.S.× 林田, 京子× ZHANG, J.× Sugimoto, C.× 井上, 昇
WEKO
63
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | The sensitivity of LAMP, PCR and microscopy to detect Theileria spp. and Trypanosoma congolense in field-derived bovine blood samples from Tanzania was evaluated and compared. No parasites were detected by microscopy. Furthermore, no bovine Theileria spp. were detected by LAMP and PCR from all the 24 samples collected from Arusha. Four and one out of 24 samples were positive for Theileria congolense infection by LAMP and PCR respectively while, 18 and nine out of 40 samples from Dar es Salaam were positive by LAMP and PCR for Theileria spp. Infection, respectively. Although all samples from Dar es Salaam were negative for Trypanosoma congolense infections by PCR, 12 out of 40 samples were LAMP positive. Whilst PCR is an established gene amplification method for the detection of Theileria and trypanosome parasites, this study introduces LAMP as an alternative molecular diagnostic tool that could be used in large-scale epidemiological surveys. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Onderstepoort Journal of Veterinary Research 巻 74, 号 4, p. 399-342, 発行日 2007 |
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出版者 | ||||||||||
言語 | en | |||||||||
出版者 | AOSIS | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 00302465 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA0076418X | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報 | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.33/> | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
日本十進分類法 | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 649 |