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Activation of HSD11B1 in the bovine cumulus-oocyte complex during IVM and IVF
https://obihiro.repo.nii.ac.jp/records/4457
https://obihiro.repo.nii.ac.jp/records/4457083753d4-2de5-4f66-9be0-f152478c40bf
名前 / ファイル | ライセンス | アクション |
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fulltext (874.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2019-10-25 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Activation of HSD11B1 in the bovine cumulus-oocyte complex during IVM and IVF | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Cumulus-oocyte complex | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Glucocorticoid | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | HSD11B1 | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | IVF | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | IVM | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
手塚, 雅文
× 手塚, 雅文
WEKO
278
× Tanakadate, Misato |
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | The bovine cumulus-oocyte complex (COC) is capable of converting cortisone, an inert glucocorticoid to active cortisol. This mechanism is mediated by 11β-hydroxysteroid oxidoreductase type 1 (HSD11B1), whose expression dramatically increases in the mature COC. In this study, we investigate the time course expression of HSD11B1 and the enzyme activity in the bovine COC undergoing maturation and fertilization in relation to key events taking place in the COC. Bovine COCs were subjected to in vitro maturation (IVM) and fertilization (IVF). The activities of HSD11B1 and HSD11B2, which mediates the opposite reaction, were measured using a radiometric conversion assay. In parallel studies, cumulus expansion, P4 production and the expression of genes associated with ovulation were measured. The reductive activity of HSD11B1 increased in the latter half of IVM and remained high during IVF, whereas the oxidative activity of HSD11B2 remained unchanged over both periods. Consequently, the net glucocorticoid metabolism in the bovine COC shifted from inactivation to activation around the time of ovulation and fertilization. The increase in HSD11B1 expression lagged behind that of P4 increase and cumulus expansion but ahead of the expressions of genes responsible for PGE2 synthesis. The reductive activity of HSD11B1 was well correlated with the cumulus expansion rate. This outcome indicates that the ability of the cumulus to activate glucocorticoids is related to its ability to synthesize hyaluronan. These results also indicate that the activation of HSD11B1 is an integral part of the sequential events taking place at the ovulation and fertilization in the bovine COC. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Endocrine Connections 巻 8, 号 7, p. 1029-1039, 発行日 2019-07 |
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出版者 | ||||||||||
言語 | en | |||||||||
出版者 | BioScientifica | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 20493614 | |||||||||
DOI | ||||||||||
関連タイプ | isIdenticalTo | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | info:doi/10.1530/EC-19-0188 | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報 | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.6/> | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
日本十進分類法 | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 645 |