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Bovine leukemia virus genotype 1 and 6 are circulating among dairy and beef cattle of small and medium holding farms in northern Vietnam
https://obihiro.repo.nii.ac.jp/records/4371
https://obihiro.repo.nii.ac.jp/records/437152be214a-6c25-435a-9f83-d5f736d66018
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2019-06-13 | |||||||
| タイトル | ||||||||
| タイトル | Bovine leukemia virus genotype 1 and 6 are circulating among dairy and beef cattle of small and medium holding farms in northern Vietnam | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Bovine leukemia virus | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Genotype | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Gp51 | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Subgenotype | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Vietnam | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 著者 |
Dao, Tung Duy
× Dao, Tung Duy× Nguyen, Hoa Thanh× Than, Son The× Bui, Vuong Nghia× 小川, 晴子× Imai, Kunitoshi
WEKO
328
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | The aims of this study were to determine the prevalence of bovine leukemia virus (BLV) infection among cattle in northern Vietnam, and to molecularly characterize the detected strains. Ninety-three blood samples (45 dairy and 48 beef cattle) were randomly collected from 464 cattle from 22 farms in four districts in northern Vietnam. The samples were tested for the presence of BLV by using two realtime PCR detection systems that targeted the BLV pol gene and tax gene. The samples were also tested for BLV antibody, using an ELISA kit. Nested PCR was designed to obtain the full-length nucleotide sequence of the BLV env gp51 gene. From the BLV pol gene real-time PCR and ELISA results, 33 of the 93 (35.48%) samples consistently tested positive for BLV infection. Phylogenetic analysis of the BLV env gp51 gene showed that the Vietnam strains were clustered into genotypes 1 and 6 (G1 and G6), of which G1 was dominant. Of three G6 strains detected, two were clearly distinguished from the known subgenotypes G6a-G6e and were therefore designated as a new subgenotype, G6f. Results of the phylogenetic analyses highlighted the requirement of the full-length env gp51 gene for reliable identification of the G6 strains. It was also noted that the commercial real-time PCR kit for the BLV tax gene failed to detect the Vietnam G6 strains, as was likewise previously reported for Myanmar BLV G10 strains. © 2019, Hokkaido University. All rights reserved. | |||||||
| 言語 | en | |||||||
| 書誌情報 |
en : Japanese Journal of Veterinary Research 巻 67, 号 1, p. 83-92, 発行日 2019-07 |
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| 出版者 | ||||||||
| 出版者 | Hokkaido University | |||||||
| 言語 | en | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 00471917 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA0069128X | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | info:doi/10.14943/jjvr.67.1.83 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
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| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 日本十進分類法 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 649 | |||||||
