Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2018-03-14 |
タイトル |
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言語 |
en |
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タイトル |
Babesia bovis BOV57, a Theileria parva P67 homolog, is an invasion-related, neutralization-sensitive antigen |
言語 |
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言語 |
eng |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Babesia bovis |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
BOV57 |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Genetic diversity |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Neutralization sensitive |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
著者 |
Ishizaki, Takahiro
Sivakumar, Thillaiampalam
林田, 京子
Takemae, Hitoshi
Tuvshintulga, Bumduuren
Munkhjargal, Tserendorj
Guswanto, Azirwan
五十嵐, 郁男
横山, 直明
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抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Babesia bovis BOV57, which is a homolog of the Theileria parva vaccine candidate antigen P67, is expressed in both the tick and blood stages of the life cycle of this parasite. However, the vaccine potential of BOV57 remained to be investigated. In the present study, we generated recombinant BOV57 (rBOV57) and prepared polyclonal antibodies against rBOV57 in mice and rabbits. Indirect immunofluorescence assays conducted with the mouse anti-rBOV57 antibody demonstrated that BOV57 localized at the apical end of paired merozoites in infected bovine red blood cells, whereas the antigen was found in the parasite membrane around the apical end of intraerythrocytic single and extracellular merozoites. In an invasion-inhibition assay, the rabbit anti-rBOV57 antibody potentially inhibited RBC invasion of B. bovis merozoites in vitro. In addition, the invasion inhibition mediated by rabbit anti-rBOV57 antibody resulted in a reduced growth rate of B. bovis in the in vitro culture. These findings indicated that B. bovis BOV57 plays a critical role in the invasion of merozoites into red blood cells, suggesting its potential as a subunit vaccine candidate against B. bovis infection in cattle. Furthermore, we analyzed the genetic diversity of bov57 gene sequences isolated from Sri Lanka, Mongolia, the Philippines, and Vietnam. The bov57 gene sequences derived from Mongolia, the Philippines, and Vietnam were conserved, whereas insertion and/or deletion mutations resulted in sequence diversity among the Sri Lankan samples. In summary, BOV57 is an invasion-related, neutralization-sensitive antigen encoded by the bov57 gene, which displays higher sequence diversity than previously reported. c 2016 Elsevier B.V. |
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言語 |
en |
書誌情報 |
en : Infection, Genetics and Evolution
巻 54,
p. 138-145,
発行日 2017
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出版者 |
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言語 |
en |
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出版者 |
Elsevier |
ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
15671348 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11697619 |
DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.1016/j.meegid.2017.06.025 |
権利 |
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権利情報 |
This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.0/> |
フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
日本十進分類法 |
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主題Scheme |
NDC |
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主題 |
649 |