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Transcriptional profiling of Toll-like receptor 2-deficient primary murine brain cells during Toxoplasma gondii infection
https://obihiro.repo.nii.ac.jp/records/4149
https://obihiro.repo.nii.ac.jp/records/4149b28a7a0f-63fe-4cfb-b5bd-f65fca9f2e0a
| 名前 / ファイル | ライセンス | アクション |
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2018-02-19 | |||||||||
| タイトル | ||||||||||
| タイトル | Transcriptional profiling of Toll-like receptor 2-deficient primary murine brain cells during Toxoplasma gondii infection | |||||||||
| 言語 | en | |||||||||
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| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Umeda, Kousuke
× Umeda, Kousuke× Tanaka, Sachi× Ihara, Fumiaki× Yamagishi, Junya× Suzuki, Yutaka× 西川, 義文
WEKO
176
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Background Toxoplasma gondii is capable of persisting in the brain, although it is efficiently eliminated by cellular immune responses in most other sites. While Toll-like receptor 2 (TLR2) reportedly plays important roles in protective immunity against the parasite, the relationship between neurological disorders induced by T. gondii infection and TLR2 function in the brain remains controversial with many unknowns. In this study, primary cultured astrocytes, microglia, neurons, and peritoneal macrophages obtained from wild-type and TLR2-deficient mice were exposed to T. gondii tachyzoites. To characterize TLR2-dependent functional pathways activated in response to T. gondii infection, gene expression of different cell types was profiled by RNA sequencing. Results During T. gondii infection, a total of 611, 777, 385, and 1105 genes were upregulated in astrocytes, microglia, neurons, and macrophages, respectively, while 163, 1207, 158, and 1274 genes were downregulated, respectively, in a TLR2-dependent manner. Overrepresented Gene Ontology (GO) terms for TLR2-dependently upregulated genes were associated with immune and stress responses in astrocytes, immune responses and developmental processes in microglia, metabolic processes and immune responses in neurons, and metabolic processes and gene expression in macrophages. Overrepresented GO terms for downregulated genes included ion transport and behavior in astrocytes, cell cycle and cell division in microglia, metabolic processes in neurons, and response to stimulus, signaling and cell motility in macrophages. Conclusions To our knowledge, this is the first transcriptomic study of TLR2 function across different cell types during T. gondii infection. Results of RNA-sequencing demonstrated roles for TLR2 varied by cell type during T. gondii infection. Our findings facilitate understanding of the detailed relationship between TLR2 and T. gondii infection, and elucidate mechanisms underlying neurological changes during infection. |
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| 言語 | en | |||||||||
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| 内容記述タイプ | Other | |||||||||
| 内容記述 | Supporting information is available at the original journal website. https://doi.org/10.1371/journal.pone.0187703 | |||||||||
| 書誌情報 |
en : PLOS ONE 巻 12, 号 11, p. e0187703, 発行日 2017-11-14 |
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| 出版者 | ||||||||||
| 出版者 | Public Library of Science | |||||||||
| 言語 | en | |||||||||
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| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 19326203 | |||||||||
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| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | PMID | |||||||||
| 関連識別子 | 29136637 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | info:doi/10.1371/journal.pone.0187703 | |||||||||
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| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
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| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 日本十進分類法 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 649 | |||||||||
