WEKO3
アイテム
{"_buckets": {"deposit": "08fbfe10-ea8e-4a5c-a6f8-781feb6a432c"}, "_deposit": {"created_by": 12, "id": "352", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "352"}, "status": "published"}, "_oai": {"id": "oai:obihiro.repo.nii.ac.jp:00000352", "sets": ["5"]}, "author_link": ["869", "867", "863", "23", "865", "864", "868", "866"], "item_2_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "271", "bibliographicPageStart": "263", "bibliographicVolumeNumber": "109", "bibliographic_titles": [{"bibliographic_title": "Acta Neuropathologica", "bibliographic_titleLang": "en"}]}]}, "item_2_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "For immunohistochemistry of the prion diseases, several pretreatment methods to enhance the immunoreactivity of human and animal abnormal proteinase-resistant prion protein (PrPSc) on the tissue sections have been employed. The method of 121 degrees C hydrated autoclaving pretreatment or the combination method of 121 degrees C hydrated autoclaving with a certain chemical reagent ( formic acid or proteinase K, etc) are now widely used. We found that an improved hydrated autoclaving method at 135 degrees C, more effectively enhanced PrPSc immunoreactivity for the antibodies recognizing the linear epitope. In addition, this method was more effective for the long-term fixation samples as compared with other previous methods. However, this modified method could not retrieve PrPSc antigenic epitopes composed of conformational structures or several discontinuous epitopes. We describe the comparative studies between our improved method and other antigen-retrieval procedures reported previously. Based on the differences of reaction among the antibodies, we also discuss the mechanisms of the hydrated autoclaving methods to retrieve PrPSc immunoreactivity.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer", "subitem_publisher_language": "en"}]}, "item_2_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Springer", "subitem_rights_language": "en"}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508984", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00016322", "subitem_source_identifier_type": "PISSN"}]}, "item_2_text_25": {"attribute_name": "文献提供者", "attribute_value_mlt": [{"subitem_text_value": "古岡秀文(furuoka@obihiro.ac.jp)"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Submitted by リポジトリ管理者 repo (repo@obihiro.ac.jp) on 2007-02-02T07:29:17Z\nNo. of bitstreams: 1\nF070202.pdf: 4688881 bytes, checksum: c6b30aac2f03b555b504c5bc1e51cda7 (MD5)"}, {"subitem_textarea_value": "Made available in DSpace on 2007-02-02T07:29:17Z (GMT). No. of bitstreams: 1\nF070202.pdf: 4688881 bytes, checksum: c6b30aac2f03b555b504c5bc1e51cda7 (MD5)\n Previous issue date: 2005-04"}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Furuoka, Hidefumi", "creatorNameLang": "en"}, {"creatorName": "古岡, 秀文", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Furuoka", "familyNameLang": "en"}, {"familyName": "古岡", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Hidefumi", "givenNameLang": "en"}, {"givenName": "秀文", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "23", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000060238665", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000060238665"}, {"nameIdentifier": "60238665", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60238665"}, {"nameIdentifier": "read0167488", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0167488"}]}, {"creatorNames": [{"creatorName": "Yabuzoe, Atushi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "863", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Horiuchi, Motohiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "864", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tagawa, Yuichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "865", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokoyama, Takashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "866", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamakawa, Yoshio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "867", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shinagawa, Morikazu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "868", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sata, Tetsutaro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "869", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "F070202.pdf", "filesize": [{"value": "4.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 4700000.0, "url": {"label": "F070202.pdf", "url": "https://obihiro.repo.nii.ac.jp/record/352/files/F070202.pdf"}, "version_id": "48cd5c69-8785-4d39-94b9-f7c8fa4eb23f"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "prion protein", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "immunohistochemistry", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "antigen retrieval", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "autoclaving", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "monoclonal antibody", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CREUTZFELDT-JAKOB-DISEASE", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "MONOCLONAL-ANTIBODIES", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IMMUNOCYTOCHEMISTRY", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IMMUNOREACTIVITY", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PROTOCOLS", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "FIXATION", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DEPOSITS", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PLAQUES", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "BRAIN", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PRP", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["5"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/352", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-02-02"}, "publish_date": "2007-02-02", "publish_status": "0", "recid": "352", "relation": {}, "relation_version_is_last": true, "title": ["Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals"], "weko_shared_id": -1}
Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals
https://obihiro.repo.nii.ac.jp/records/352
https://obihiro.repo.nii.ac.jp/records/352d86ae9b3-e9a3-451a-9fe7-64ede99b6892
名前 / ファイル | ライセンス | アクション |
---|---|---|
F070202.pdf (4.7 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2007-02-02 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | prion protein | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | immunohistochemistry | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | antigen retrieval | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | autoclaving | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | monoclonal antibody | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | CREUTZFELDT-JAKOB-DISEASE | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | MONOCLONAL-ANTIBODIES | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | IMMUNOCYTOCHEMISTRY | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | IMMUNOREACTIVITY | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | PROTOCOLS | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | FIXATION | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | DEPOSITS | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | PLAQUES | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | BRAIN | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | PRP | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
古岡, 秀文
× 古岡, 秀文
WEKO
23
× Yabuzoe, Atushi× Horiuchi, Motohiro× Tagawa, Yuichi× Yokoyama, Takashi× Yamakawa, Yoshio× Shinagawa, Morikazu× Sata, Tetsutaro |
|||||||||
抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | For immunohistochemistry of the prion diseases, several pretreatment methods to enhance the immunoreactivity of human and animal abnormal proteinase-resistant prion protein (PrPSc) on the tissue sections have been employed. The method of 121 degrees C hydrated autoclaving pretreatment or the combination method of 121 degrees C hydrated autoclaving with a certain chemical reagent ( formic acid or proteinase K, etc) are now widely used. We found that an improved hydrated autoclaving method at 135 degrees C, more effectively enhanced PrPSc immunoreactivity for the antibodies recognizing the linear epitope. In addition, this method was more effective for the long-term fixation samples as compared with other previous methods. However, this modified method could not retrieve PrPSc antigenic epitopes composed of conformational structures or several discontinuous epitopes. We describe the comparative studies between our improved method and other antigen-retrieval procedures reported previously. Based on the differences of reaction among the antibodies, we also discuss the mechanisms of the hydrated autoclaving methods to retrieve PrPSc immunoreactivity. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Acta Neuropathologica 巻 109, 号 3, p. 263-271, 発行日 2005-04 |
|||||||||
出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Springer | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 00016322 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA00508984 | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報 | Springer | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |