| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-02-20 |
| タイトル |
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|
タイトル |
Neospora caninum surface antigen 1 is a major determinant of the pathogenesis of neosporosis in nonpregnant and pregnant mice |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Balb/c mouse |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CRISPR/Cas9 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gene editing |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NcSAG1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Neospora caninum |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
pregnancy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
vertical transmission |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
ABDELBAKY, Hanan Hassan Mahmoud
Md. Masudur, Rahman
Shimoda, Naomi
Chen, Yu
Hasan, Tanjila
Ushio, Nanako
西川, 義文
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Introduction: NcSAG1 is one of most widely investigated antigens of Neospora caninum in various research fields. Such studies demonstrated the proficiency of NcSAG1 in the regulatory process of parasite adhesion and invasion of host cells. Accordingly, the contribution of NcSAG1 to the pathogenesis of neosporosis can undoubtedly be extrapolated, but direct evidence is lacking. Herein, we provide the first successful attempt at the gene disruption of NcSAG1 and novel data on the invasion and virulence potentials of N. caninum in vitro and in vivo. Methods: The disruption of the NcSAG1 gene was applied using the CRISPR/Cas9 system and confirmed by PCR, western blot and indirect fluorescent antibody tests as NcSAG1 knockout parasites (NcSAG1KO). Then, we investigated the role of NcSAG1 in the growth kinetics of the parasite in vitro. Results and discussion: The deletion of the NcSAG1 gene significantly decreased the infection rate and reduced the egress rate of the parasite. An in vivo study using nonpregnant female and male BALB/c mice revealed a significantly higher survival rate and lower body weight change in the group infected with the NcSAG1KO parasite than in the parental strain (Nc-1)-infected group. Regarding the vertical transmission model of BALB/c mice, the absence of the NcSAG1 gene significantly enhanced the survival of pups and greatly lowered the parasite burden in the brains of pups. In conclusion, our study suggested NcSAG1 as a key molecule in the pathogenesis of N. caninum. Copyright © 2024 Abdelbaky, Rahman, Shimoda, Chen, Hasan, Ushio and Nishikawa. |
|
言語 |
en |
| 書誌情報 |
en : Frontiers in Microbiology
巻 14,
p. 1334447,
発行日 2024-01-08
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| 出版者 |
|
|
出版者 |
Frontiers Media SA |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
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収録物識別子 |
1664302X |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.3389/fmicb.2023.1334447 |
| フォーマット |
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|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
言語 |
ja |
|
主題Scheme |
NDC |
|
主題 |
649 |
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