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Role of Chicken Pancreatic Trypsin, Chymotrypsin and Elastase in the Excystation Process of Eimeria tenella Oocysts and Sporocysts
https://obihiro.repo.nii.ac.jp/records/1728
https://obihiro.repo.nii.ac.jp/records/172813b23b87-2231-4866-b854-78c73d9f9e9b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Prot.Vol.1-(3).pdf (158.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-12-21 | |||||
| タイトル | ||||||
| タイトル | Role of Chicken Pancreatic Trypsin, Chymotrypsin and Elastase in the Excystation Process of Eimeria tenella Oocysts and Sporocysts | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | chymotrypsin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Eimeria tenella | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | elastase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | excystation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | trypsin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Guyonnet, Vincent
× Guyonnet, Vincent× Johnson, Joyce K.× Long, Peter L. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The role of pancreatic proteolytic enzymes in the excystation process of Eimeria tenella oocysts and sporocysts was studied in vitro. Intact sporulated oocysts were preincubated in phosphate buffer, NaCl 0.9% (PBS) added with 0.5% chicken bile extract in a 5% C02 atmosphere for 30 minutes prior to exposure to either 0.25% (w/v) chicken trypsin, chymotrypsin, pancreatic elastase, or a 1% (w/v) crude extract of unsporulated and sporulated oocysts of E. tenella (Expt.1). No excystation was observed under these conditions. Sporocysts were also incubated under the same conditions without pretreatment in C02. Excystation was observed for sporocysts incubated with either trypsin, chymotrypsin or pancreatic elastase, the best percentage of excystation being recorded for the latter after 5 hours (Expt. 2). Crude extracts of E. tenella oocysts failed to bring about excystation of sporocysts at any time. In experiment 3, sporocysts were incubated with either trypsin, chymotrypsin, or pancreatic elastase alone, any combinations of 2 of these enzymes or with all 3 enzymes. The best percentage of excystation was observed after 5 hours with sporocysts incubated with trypsin and chymotrypsin (99%). The other combinations of 2 enzymes gave also comparable results. Sporocysts incubated in the presence of the 3 enzymes excysted similarly well, although a significantly lower percentage (P<0.05) after 5 hours was recorded when compared to that in sporocysts incubated with trypsin and chymotrypsin. In most cases, the association of 2 or 3 enzymes had a synergistic effect on the percentage excystation of sporocysts in vitro. |
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| 言語 | en | |||||
| 書誌情報 |
en : The journal of protozoology research 巻 1, p. 22-26, 発行日 1991-10 |
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| 出版者 | ||||||
| 出版者 | Research Center for Protozoan Molecular Immunology | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 09174427 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10824856 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Research Center for Protozoan Molecular Immunology | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 言語 | en | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 649 | |||||
