WEKO3
アイテム
{"_buckets": {"deposit": "88357bbc-0ce6-45b8-954b-24e409fea1ab"}, "_deposit": {"created_by": 12, "id": "1518", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "1518"}, "status": "published"}, "_oai": {"id": "oai:obihiro.repo.nii.ac.jp:00001518", "sets": ["32"]}, "author_link": ["358", "58", "3291", "295", "312", "255"], "item_2_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "14", "bibliographicPageStart": "10", "bibliographicVolumeNumber": "13", "bibliographic_titles": [{"bibliographic_title": "The journal of protozoology research", "bibliographic_titleLang": "en"}]}]}, "item_2_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In hemoprotozoa,gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study,a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection,clear positive fluorescences were detected in some parasite bodies, indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However, the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that the expression of a foreign gene has been reported in the Babesia parasite.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "National Research Center for Protozoan Diseases", "subitem_publisher_language": "en"}]}, "item_2_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "National Research Center for Protozoan Diseases", "subitem_rights_language": "en"}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10824856", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09174427", "subitem_source_identifier_type": "PISSN"}]}, "item_2_subject_21": {"attribute_name": "日本十進分類法", "attribute_value_mlt": [{"subitem_subject": "649", "subitem_subject_scheme": "NDC"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Submitted by リポジトリ管理者 repo (repo@obihiro.ac.jp) on 2007-01-25T02:18:40Z\nNo. of bitstreams: 1\nProt.Vol.13-1,2-2.pdf: 860856 bytes, checksum: f98d5539df47b07d636a061fee726a77 (MD5)"}, {"subitem_textarea_value": "Made available in DSpace on 2007-01-25T02:18:40Z (GMT). No. of bitstreams: 1\nProt.Vol.13-1,2-2.pdf: 860856 bytes, checksum: f98d5539df47b07d636a061fee726a77 (MD5)\n Previous issue date: 2003-04"}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fujii, Kei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "3291", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Yokoyama, Naoaki", "creatorNameLang": "en"}, {"creatorName": "横山, 直明", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Yokoyama", "familyNameLang": "en"}, {"familyName": "横山", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Naoaki", "givenNameLang": "en"}, {"givenName": "直明", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "312", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000002627114", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000002627114"}, {"nameIdentifier": "80301802", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80301802"}, {"nameIdentifier": "read0074761", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0074761"}]}, {"creatorNames": [{"creatorName": "Takabatake, Noriyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "358", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006555593", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006555593"}]}, {"creatorNames": [{"creatorName": "Suzuki, Hiroshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "255", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000003006849", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000003006849"}, {"nameIdentifier": "60333473", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60333473"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Xuan, Xuenan", "creatorNameLang": "en"}, {"creatorName": "玄, 学南", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Xuan", "familyNameLang": "en"}, {"familyName": "玄", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Xuenan", "givenNameLang": "en"}, {"givenName": "学南", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "295", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000002841347", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000002841347"}, {"nameIdentifier": "10292096", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=10292096"}, {"nameIdentifier": "read0055834", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0055834"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Igarashi, Ikuo", "creatorNameLang": "en"}, {"creatorName": "五十嵐, 郁男", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Igarashi", "familyNameLang": "en"}, {"familyName": "五十嵐", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Ikuo", "givenNameLang": "en"}, {"givenName": "郁男", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "58", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000003078567", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000003078567"}, {"nameIdentifier": "80159582", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80159582"}, {"nameIdentifier": "read0167555", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0167555"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "1209.pdf", "filesize": [{"value": "927.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 927100.0, "url": {"label": "1209.pdf", "url": "https://obihiro.repo.nii.ac.jp/record/1518/files/1209.pdf"}, "version_id": "24eeadf1-c063-43a4-80f6-5a797e37804d"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["32"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/1518", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-01-25"}, "publish_date": "2007-01-25", "publish_status": "0", "recid": "1518", "relation": {}, "relation_version_is_last": true, "title": ["TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS"], "weko_shared_id": -1}
TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS
名前 / ファイル | ライセンス | アクション |
---|---|---|
1209.pdf (927.1 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2007-01-25 | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | en | |||||||||||||||||
タイトル | TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | eng | |||||||||||||||||
資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | journal article | |||||||||||||||||
著者 |
Fujii, Kei
× Fujii, Kei× 横山, 直明
WEKO
312
× Takabatake, Noriyuki× Suzuki, Hiroshi× 玄, 学南
WEKO
295
× 五十嵐, 郁男
WEKO
58
|
|||||||||||||||||
抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | In hemoprotozoa,gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study,a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection,clear positive fluorescences were detected in some parasite bodies, indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However, the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that the expression of a foreign gene has been reported in the Babesia parasite. | |||||||||||||||||
言語 | en | |||||||||||||||||
書誌情報 |
en : The journal of protozoology research 巻 13, 号 1-2, p. 10-14, 発行日 2003-04 |
|||||||||||||||||
出版者 | ||||||||||||||||||
言語 | en | |||||||||||||||||
出版者 | National Research Center for Protozoan Diseases | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 09174427 | |||||||||||||||||
書誌レコードID | ||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AA10824856 | |||||||||||||||||
権利 | ||||||||||||||||||
言語 | en | |||||||||||||||||
権利情報 | National Research Center for Protozoan Diseases | |||||||||||||||||
フォーマット | ||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||
著者版フラグ | ||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
日本十進分類法 | ||||||||||||||||||
主題Scheme | NDC | |||||||||||||||||
主題 | 649 |