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Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites
| 名前 / ファイル | ライセンス | アクション |
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gen070711-2.pdf (604.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2007-07-11 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Neospora caninum | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Toxoplasma gondii | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | apical membrane antigen 1 | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | cross-reactive | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | invasion | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| 著者 |
Zhang, Houshuang
× Zhang, Houshuang× Compaore, Muller K.A.× Lee, Eung-goo× Liao, Min× Zhang, Guohong× Sugimoto, Chihiro
WEKO
252
× Fujisaki, Kozo× 西川, 義文
WEKO
176
× 玄, 学南
WEKO
295
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by Western blotting. Immunofluorescence analysis showed that NcAMA1 was localized to the apical end of tachyzoites. Two-dimensional electrophoresis and Western blotting indicated that an approximately 57-kDa cleavage product was released into the excretory/secretory products of N. caninum. Preincubation of free tachyzoites with anti-rNcAMA1 IgG antibodies inhibited the invasion into host cells by N. caninum and T gondii. These results indicated that AMA1 is a cross-reactive antigen between N. caninum and T gondii and a potential common vaccine candidate to control two parasites. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 書誌情報 |
en : Molecular and Biochemical Parasitology 巻 151, 号 2, p. 205-212, 発行日 2007-02 |
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| 出版者 | ||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||
| 言語 | en | |||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||
| 収録物識別子 | 01666851 | |||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||
| 収録物識別子 | AA00307472 | |||||||||||||||
| 権利 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 権利情報 | Elsevier | |||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
| 日本十進分類法 | ||||||||||||||||
| 主題Scheme | NDC | |||||||||||||||
| 主題 | 649 | |||||||||||||||
