@article{oai:obihiro.repo.nii.ac.jp:00000502,
 author = {Zhang, Houshuang and Compaore, Muller K.A. and Lee, Eung-goo and Liao, Min and Zhang, Guohong and Sugimoto, Chihiro and Fujisaki, Kozo and Nishikawa, Yoshifumi and 西川, 義文 and Xuan, Xuenan and 玄, 学南},
 issue = {2},
 journal = {Molecular and Biochemical Parasitology},
 month = {Feb},
 note = {application/pdf, The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by Western blotting. Immunofluorescence analysis showed that NcAMA1 was localized to the apical end of tachyzoites. Two-dimensional electrophoresis and Western blotting indicated that an approximately 57-kDa cleavage product was released into the excretory/secretory products of N. caninum. Preincubation of free tachyzoites with anti-rNcAMA1 IgG antibodies inhibited the invasion into host cells by N. caninum and T gondii. These results indicated that AMA1 is a cross-reactive antigen between N. caninum and T gondii and a potential common vaccine candidate to control two parasites.},
 pages = {205--212},
 title = {Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites},
 volume = {151},
 year = {2007}
}