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Evaluating the use of piezo manipulator, laser or their combination for blastocoel cavity puncture to improve cryopreservation outcomes of large equine embryos
https://obihiro.repo.nii.ac.jp/records/4844
https://obihiro.repo.nii.ac.jp/records/48449088bcfd-642d-45f9-a6d1-8096193c0742
名前 / ファイル | ライセンス | アクション |
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Full text (700.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2023-03-16 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Evaluating the use of piezo manipulator, laser or their combination for blastocoel cavity puncture to improve cryopreservation outcomes of large equine embryos | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | blastocoel fluid, cryopreservation, cryoprotectant, embryo, equine, laser pulse, piezo manipulator | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Rajabi-Toustani, Reza
× Rajabi-Toustani, Reza× Watanabe, Hiroyuki× Tsogtgerel, Munkhtuul× Gao, Yuanzhi× Canbo, Li× 羽田, 真悟
WEKO
32
× Cheong, Soon Hon× 南保, 泰雄
WEKO
171
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | The main difficulty of large equine embryo cryopreservation is the replacement of blastocoel fluid with cryoprotectant solution. The objective of this study was to improve the cryopreservation of large equine embryos with PMAP and/or LAP. Embryos were collected via the non-surgical transcervical procedure and divided into three groups based on their size (A ≤ 300 µm, 300 µm<B < 700 µm and C ≥ 700 µm). Six embryos 233–1360 µm in diameter were punctured via piezo manipulator and/or laser pulse before cryopreservation. All embryos were cryopreserved on a Cryotop®. Frozen-thawed embryos were cultured for 3h and transferred to the recipient mares. After one week, pregnancy was diagnosed by ultrasonography. Two of six embryos resulted in a positive pregnancy, the result of pregnancy in group A and B was positive, but in group C was negative, and further investigation is necessary for ≥700 µm embryos. The results showed laser-assisted puncture could be helpful to extract blastocoel fluid and replace it with cryoprotectant. This is the first positive pregnancy report in laser puncture-assisted frozen-thawed equine embryo (>300 µm). However, more research is required to find the best method for embryos ≥700 µm. | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : Reproduction in Domestic Animals 巻 56, 号 10, p. 1358-1362, 発行日 2021-07-13 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | John Wiley | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 0936-6768 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA10748995 | |||||||||||||
DOI | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | info:doi/10.1111/rda.13990 | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | AM | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
日本十進分類法 | ||||||||||||||
主題Scheme | NDC | |||||||||||||
主題 | 649 |