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Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa
https://obihiro.repo.nii.ac.jp/records/1075
https://obihiro.repo.nii.ac.jp/records/10751890839f-f47a-4e03-adee-71d90623f213
名前 / ファイル | ライセンス | アクション |
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inoue77(2),217-220,2015.pdf (708.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2015-03-27 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | animal African trypanosomosis | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | ELISA | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | immunochromatographic test | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | serodiagnosis | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | South Africa | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Nguyen, Thu-Thuy
× Nguyen, Thu-Thuy× Motsiri, Mono Sophie× Taioe, Moeti Oriel× Mtshali, Moses Sibusiso× Goto, Yasuyuki× 河津, 信一郎× Molifi, Thekisoe Oriel Matlhahane× 井上, 昇
WEKO
63
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6–0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Journal of Veterinary Medical Science 巻 77, 号 2, p. 217-220, 発行日 2015 |
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出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Japanese Society of Veterinary Science | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 09167250 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA10796138 | |||||||||
DOI | ||||||||||
関連タイプ | isIdenticalTo | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | info:doi/10.1292/jvms.14-0330 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |