@article{oai:obihiro.repo.nii.ac.jp:00001075,
 author = {Nguyen, Thu-Thuy and Motsiri, Mono Sophie and Taioe, Moeti Oriel and Mtshali, Moses Sibusiso and Goto, Yasuyuki and Kawazu, Shin-ichiro and 河津, 信一郎 and Molifi, Thekisoe  Oriel Matlhahane and Inoue, Noboru and 井上, 昇},
 issue = {2},
 journal = {Journal of Veterinary Medical Science},
 month = {},
 note = {application/pdf, A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6–0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.},
 pages = {217--220},
 title = {Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa},
 volume = {77},
 year = {2015}
}