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常温性菌核病菌 Sclerotinia sclerotiorum 由来のポリガラクツロナーゼIの単離
https://obihiro.repo.nii.ac.jp/records/1852
https://obihiro.repo.nii.ac.jp/records/18528cc7e8f4-a84f-4e20-aa1c-4b4e8a6e5e25
名前 / ファイル | ライセンス | アクション |
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t061122-1.pdf (642.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-11-22 | |||||
タイトル | ||||||
タイトル | 常温性菌核病菌 Sclerotinia sclerotiorum 由来のポリガラクツロナーゼIの単離 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sclerotinia sclerotiorum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 常温菌 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 菌核病菌 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ポリガラクツロナーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 細胞壁分解酵素 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mesophile | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | white mold | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polygalacturonase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cell wall degrading enzyme | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Isolation of polygalacturonase I from the culture of the mesophilic white mold Sclerotinia sclerotiorum | |||||
著者 |
高橋, 裕司
× 高橋, 裕司× 井熊, 武志× 匂坂, 慶子× 斉藤, 泉× 高澤, 俊英 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | CM-Toyopearl陽イオン交換カラムクロマトグラフィーおよびSephacryl S-200ゲルろ過カラムクロマトグラフィーによって、常温性菌核病菌Sclerotinia sclerotiorumの20℃培養抽出物のポリガラクツロナーゼ活性画分からポリガラクツロナーゼ I を単離した。ポリガラクツロナーゼ I 精製標品(P4-4-2)の比活性は3662U/mgであり、粗抽出液から全体として116倍に精製した。精製標品はポリアクリルアミドゲル電気泳動によって、均一であることが示された。また、SDS存在下ポリアクリルアミドゲル電気泳動によって本酵素の分子質量は39.8±1.10kDa(S.E.)、さらに等電点電気泳動によって本酵素の等電点はpH8.70と見積もられた。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Polygaracturonase I has been isolated from the mesophilic white mold Sclerotinia Sclerotiorum by cation-exchange chromatography on a column of CM-Toyopearl followed by gel filtration on a column of Sephacryl S-200. Purified enzyme preparation(P4-4-2) had a specific activity of 3 662 units per mg of protein, that is, 116-fold purification over the crude extract of the wheat bran culture of the mesophilic fungus S. sclerotiorum. The purified enzyme was determined to be homogeneous by nondenaturing polyacrylamide gel electrophoresis. The molecular mass of the enzyme was estimated to be 39.8±1.1kDa(S.E.) by the polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and its isoelectric point was estimated to be pH 8.70 by isoelectric focusing polyacrylamide gel electrophoresis. | |||||
書誌情報 |
帯広畜産大学学術研究報告. 自然科学 en : Research bulletin of Obihiro University. Natural science 巻 23, 号 1, p. 7-17, 発行日 2002-12 |
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出版者 | ||||||
出版者 | 帯広畜産大学 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13476556 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11877530 | |||||
権利 | ||||||
権利情報 | 帯広畜産大学 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |