{"created":"2023-05-15T15:16:06.313840+00:00","id":1852,"links":{},"metadata":{"_buckets":{"deposit":"837d4e58-43c9-4125-8fbd-3ad9278785fe"},"_deposit":{"created_by":3,"id":"1852","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1852"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00001852","sets":["74:91:92:94"]},"author_link":["211","60","268","270","209"],"item_5_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Isolation of polygalacturonase I from the culture of the mesophilic white mold Sclerotinia sclerotiorum"}]},"item_5_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"17","bibliographicPageStart":"7","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"帯広畜産大学学術研究報告. 自然科学"},{"bibliographic_title":"Research bulletin of Obihiro University. Natural science","bibliographic_titleLang":"en"}]}]},"item_5_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CM-Toyopearl陽イオン交換カラムクロマトグラフィーおよびSephacryl S-200ゲルろ過カラムクロマトグラフィーによって、常温性菌核病菌Sclerotinia sclerotiorumの20℃培養抽出物のポリガラクツロナーゼ活性画分からポリガラクツロナーゼ I を単離した。ポリガラクツロナーゼ I 精製標品(P4-4-2)の比活性は3662U/mgであり、粗抽出液から全体として116倍に精製した。精製標品はポリアクリルアミドゲル電気泳動によって、均一であることが示された。また、SDS存在下ポリアクリルアミドゲル電気泳動によって本酵素の分子質量は39.8±1.10kDa(S.E.)、さらに等電点電気泳動によって本酵素の等電点はpH8.70と見積もられた。","subitem_description_type":"Abstract"},{"subitem_description":"Polygaracturonase I has been isolated from the mesophilic white mold Sclerotinia Sclerotiorum by cation-exchange chromatography on a column of CM-Toyopearl followed by gel filtration on a column of Sephacryl S-200. Purified enzyme preparation(P4-4-2) had a specific activity of 3 662 units per mg of protein, that is, 116-fold purification over the crude extract of the wheat bran culture of the mesophilic fungus S. sclerotiorum. The purified enzyme was determined to be homogeneous by nondenaturing polyacrylamide gel electrophoresis. The molecular mass of the enzyme was estimated to be 39.8±1.1kDa(S.E.) by the polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and its isoelectric point was estimated to be pH 8.70 by isoelectric focusing polyacrylamide gel electrophoresis.","subitem_description_type":"Abstract"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"帯広畜産大学"}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"帯広畜産大学"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11877530","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13476556","subitem_source_identifier_type":"ISSN"}]},"item_5_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高橋, 裕司"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Ikuma, Takeshi","creatorNameLang":"en"},{"creatorName":"井熊, 武志","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"匂坂, 慶子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"斉藤, 泉"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"高澤, 俊英"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"t061122-1.pdf","filesize":[{"value":"642.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"t061122-1.pdf","url":"https://obihiro.repo.nii.ac.jp/record/1852/files/t061122-1.pdf"},"version_id":"09b508f7-98f9-4af7-9b54-c970bbf61e08"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Sclerotinia sclerotiorum","subitem_subject_scheme":"Other"},{"subitem_subject":"常温菌","subitem_subject_scheme":"Other"},{"subitem_subject":"菌核病菌","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリガラクツロナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞壁分解酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"mesophile","subitem_subject_scheme":"Other"},{"subitem_subject":"white mold","subitem_subject_scheme":"Other"},{"subitem_subject":"polygalacturonase","subitem_subject_scheme":"Other"},{"subitem_subject":"cell wall degrading enzyme","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"常温性菌核病菌 Sclerotinia sclerotiorum 由来のポリガラクツロナーゼIの単離","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"常温性菌核病菌 Sclerotinia sclerotiorum 由来のポリガラクツロナーゼIの単離"}]},"item_type_id":"5","owner":"3","path":["94"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-11-22"},"publish_date":"2006-11-22","publish_status":"0","recid":"1852","relation_version_is_last":true,"title":["常温性菌核病菌 Sclerotinia sclerotiorum 由来のポリガラクツロナーゼIの単離"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-12-07T04:34:21.208909+00:00"}