@article{oai:obihiro.repo.nii.ac.jp:00000983, author = {Sivakumar, Thillaiampalam and Asada, Masahito and Battsetseg, Badgar and Huang, Xiaohong and Lan, Dinh Thi Bich and Inpankaew, Tawin and Ybañez, Adrian Patalinghug and Alhassan, Andy and Thekisoe, Oriel M. M. and De, Macedo Alan Caine Costa and Inokuma, Hisashi and 猪熊, 壽 and Igarashi, Ikuo and 五十嵐, 郁男 and Yokoyama, Naoaki and 横山, 直明}, issue = {2}, journal = {Journal of Veterinary Medical Science}, month = {}, note = {application/pdf, Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B. ovata infection in Japan, Mongolia, and Thailand.}, pages = {211--214}, title = {A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African and South American Countries}, volume = {75}, year = {2013} }