@article{oai:obihiro.repo.nii.ac.jp:00000955,
 author = {Ybañez, Adrian Patalinghug and Sivakumar, Thillaiampalam and Ybañez, Rochelle Haidee Daclan and Ratilla, Jowarren Catingan and Perez, Zandro Obligado and Gabotero, Shirleny Reyes and Hakimi, Hassan and Kawazu, Shin-ichiro and 河津, 信一郎 and Matsumoto, Kohtaro and 松本, 高太郎 and Yokoyama, Naoaki and 横山, 直明 and Inokuma, Hisashi and 猪熊, 壽},
 issue = {1},
 journal = {Journal of Veterinary Medical Science},
 month = {},
 note = {application/pdf, Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale from the Philippines revealed 99.1–100% (16S rRNA, gltA, groEL and Msp5) and 94.3–97.6% (Msp1α) identities to the closest isolates from other countries. Moreover, sequence analysis of the Msp1α, gene showed 3 variants, including a case of co-infection with 2 variants. Phylogenetic analyses based on Msp1α and Msp5 genes revealed that Philippine A. marginale isolates formed a monophyletic lineage, and were phylogenetically related to Brazilian and Chinese isolates. On the other hand, a highly specific and sensitive nested PCR based on groEL, with a detection limit of 2 copies/PCR, was developed to detect A. marginale in the Philippines. This study reported the first molecular detection and characterization of A. marginale in cattle and R. microplus in Cebu, Philippines.},
 pages = {27--36},
 title = {First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines},
 volume = {75},
 year = {2013}
}