@article{oai:obihiro.repo.nii.ac.jp:00000947, author = {Ybañez, Adrian Patalinghug and Sato, Fumio and Nambo, Yasuo and 南保, 泰雄 and Fukui, Takashi and Masuzawa, Toshiyuki and Ohashi, Norio and Matsumoto, Kohtaro and 松本, 高太郎 and Kishimoto, Toshio and Inokuma, Hisashi and 猪熊, 壽}, issue = {1}, journal = {Journal of Veterinary Medical Science}, month = {}, note = {application/pdf, A total of 87 Thoroughbred horses and 10 ixodid ticks from a ranch in Hidaka district, Hokkaido were tested for tick-borne diseases. Using the indirect fluorescent antibody (IFA) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥80 against Anaplasma phagocytophilum, Rickettsia helvetica, and Borrelia garinii, respectively. This is the first report of infection with the 3 pathogens in horses in Japan. Using PCR, DNAs from the peripheral blood of all horses were found negative with any Anaplasma, Rickettsia and Borrelia spp., while those from Haemaphysalis megaspinosa ticks were found positive for Anaplasma sp. closely related to A. phagocytophilum in Japan, and A. bovis. B. japonica was also detected in an H. flava tick for the first time.}, pages = {11--15}, title = {Survey on Tick-Borne Pathogens in Thoroughbred Horses in the Hidaka District, Hokkaido, Japan}, volume = {75}, year = {2013} }