@article{oai:obihiro.repo.nii.ac.jp:00000835,
 author = {Yokoyama, Naoaki and 横山, 直明 and Ueno, Akio and Mizuno, Daisuke and Kuboki, Noritaka and Khukhuu, Altangerel and Igarashi, Ikuo and 五十嵐, 郁男 and Miyahara, Tokuji and Shiraishi, Takashi and Kudo, Ryuta and Oshiro, Mamoru and Zakimi, Satoshi and Sugimoto, Chihiro and Matsumoto, Kohtaro and 松本, 高太郎 and Inokuma, Hisashi and 猪熊, 壽},
 issue = {3},
 journal = {Journal of Veterinary Medical Science},
 month = {},
 note = {application/pdf, Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic
losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface
protein （MPSP） and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture
（Aso and Kuma districts） and 3 farms in Okinawa prefecture （Ishigaki, Iriomote, and Yonaguni Islands）. High prevalence rates
of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses
revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore, MPSP genotype-specific PCR methods were developed in this study
and wide distributions of 5-district genotypes of T. orientalis were observed for the examined farms. Our results indicate that at least 5
types of T. orientalis exist in Kumamoto and Okinawa prefectures of Japan and that genotype-specific PCR assays are highly applicable
for the quarantine of transported cattle and for epidemiological surveys of bovine theileriosis in Japan.},
 pages = {305--312},
 title = {Genotypic Diversity of Theileria orientalis Detected from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan},
 volume = {73},
 year = {2011}
}