{"created":"2023-05-15T15:15:16.679269+00:00","id":834,"links":{},"metadata":{"_buckets":{"deposit":"7254548e-55fe-4eb5-80b3-3c19cd3f4a5e"},"_deposit":{"created_by":3,"id":"834","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"834"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000834","sets":["5"]},"author_link":["1738","295","1737","1736","1735","176"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"639","bibliographicPageStart":"633","bibliographicVolumeNumber":"73","bibliographic_titles":[{},{"bibliographic_title":"Journal of Veterinary Medical Science","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The intracellular protozoan Toxoplasma gondii lacks the ability to synthesize sterol and scavenges cholesterol from the lowdensity\nlipoprotein receptor (LDLR) pathway of its host to facilitate replication. Sterol biosynthesis inhibitors, however, have a demonstrated\nanti-Toxoplasma effect. In this study, we examined the host mevalonate pathway as a novel source of cholesterol for T. gondii\nand its effects on parasite growth in macrophages. Parasite growth did not significantly change in the absence of LDLR or when LDL\nwas exogenously supplemented. Lovastatin and compactin, both inhibitors of hydroxymethylglutaryl-CoA (HMG-CoA) reductase in the\nmevalonate pathway, significantly inhibited T. gondii growth in both wild-type and LDLR-knockout macrophages. Parasite growth was\nalso suppressed by squalestatin, an inhibitor of squalene synthase, despite mevalonate producing isoprenoid intermediates in host cells.\nThe present study demonstrates that lovastatin, compactin and squalestatin have anti-Toxoplasma activities and that the host cholesterol\nsynthesis may contribute to parasite growth in macrophages.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1292/jvms.10-0496","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Japanese Society of Veterinary Science"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10796138","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09167250","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Nishikawa, Yoshifumi","creatorNameLang":"en"},{"creatorName":"西川, 義文","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{},{}]},{"creatorNames":[{"creatorName":"Ibrahim, Hany M.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kameyama, Kyohko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiga, Ikumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hiasa, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Xuan, Xuenan","creatorNameLang":"en"},{"creatorName":"玄, 学南","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"633.pdf","filesize":[{"value":"839.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"633.pdf","url":"https://obihiro.repo.nii.ac.jp/record/834/files/633.pdf"},"version_id":"2cb5723f-d1e5-431a-ac6e-95919bf330f4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cholesterol","subitem_subject_scheme":"Other"},{"subitem_subject":"lipid metabolism","subitem_subject_scheme":"Other"},{"subitem_subject":"macrophage","subitem_subject_scheme":"Other"},{"subitem_subject":"parasitology","subitem_subject_scheme":"Other"},{"subitem_subject":"Toxoplasma gondii","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["5"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-05-21"},"publish_date":"2013-05-21","publish_status":"0","recid":"834","relation_version_is_last":true,"title":["Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-02-28T03:14:01.115369+00:00"}