{"created":"2023-05-15T15:15:14.890994+00:00","id":793,"links":{},"metadata":{"_buckets":{"deposit":"6470f40d-28bf-46c7-ac7f-596e44101511"},"_deposit":{"created_by":12,"id":"793","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"793"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000793","sets":["5"]},"author_link":["1684","255"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"182","bibliographicPageStart":"176","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"Journal of Reproduction and Development","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This review describes the study of freeze-dried mouse sperm for practical application in preserving and transporting genetic resources. Freeze-dried sperm can be used to preserve and transport genetic resources; however, there still remain many areas which need to be studied. In particular, it is essential to assure long-term preservation over several decades or centuries. Recently, the theory of accelerated degradation kinetics to freeze-dried mouse sperm has been applied, and found that long-term preservation by conventional methods requires temperatures lower than -80 C. When the relationship between the pressure at primary drying and the preservation potential of freeze-dried mouse sperm was examined, a pressure of 0.37 mbar at primary drying significantly improved the developmental rate to the blastocyst stage. In addition, it has been shown that freeze-dried sperm stored at -80 C with and without transportation can retain their ability to generate viable offspring after storage for up to 2 years. Sperm chromatin structure assay （SCSA） was applied to mouse sperm freeze-dried under several conditions and compared the results with the embryonic developmental rates of freeze-dried sperm after intracytoplasmic sperm injection （ICSI） and with comet assay results. Furthermore, SCSA might be useful for estimation of developmental potential of fertilized eggs derived from ICSI using freeze-dried sperm in mice.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"SRD Young Investigator Award","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Reproduction and Development","subitem_publisher_language":"en"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1262/jrd.10-199E","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Japanese Society of Animal Reproduction","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10936678","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09168818","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawase, Yosuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"SRD.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SRD.pdf","url":"https://obihiro.repo.nii.ac.jp/record/793/files/SRD.pdf"},"version_id":"985be31f-cd56-415a-902e-92c462e670bf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Comet assay","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Freeze-dried sperm","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ICSI","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SCSA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Study on Freeze-Drying as a Method of Preserving Mouse Sperm","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Study on Freeze-Drying as a Method of Preserving Mouse Sperm","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-07-29"},"publish_date":"2011-07-29","publish_status":"0","recid":"793","relation_version_is_last":true,"title":["A Study on Freeze-Drying as a Method of Preserving Mouse Sperm"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-28T03:00:36.702758+00:00"}