{"created":"2023-05-15T15:15:11.814911+00:00","id":728,"links":{},"metadata":{"_buckets":{"deposit":"d3688877-c365-4665-9ad0-edf02ae8cc60"},"_deposit":{"created_by":12,"id":"728","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"728"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000728","sets":["5"]},"author_link":["1555","322","1556","318"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"805","bibliographicPageStart":"795","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"Journal of Toxicological Sciences","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Botulinum neurotoxin serotype A （BoNT/A) inhibits acetylcholine release at the neuromuscular junction in isolated muscles, and ouabain can partially block its effect. However, it is not clear whether ouabain attenuates BoNT/A-induced neuromuscular paralysis in vivo. In this work, we investigated the effects of ouabain on BoNT/A-induced neuromuscular paralysis in mice. Ouabain was administered to mice intraperitoneally immediately after a single injection of BoNT/A into skeletal muscle. The effects of ouabain on BoNT/A-induced muscle paralysis were assessed by quantitative monitoring of muscle tension and digit abduction via the digit abduction scoring （DAS) assay. A single administration of ouabain significantly prolonged BoNT/A-induced neuromuscular paralysis. Moreover, consecutive daily injection of ouabain exacerbated BoNT/A-induced neuromuscular paralysis, and led to a significant decrease in both twitch and tetanic forces as assayed in isolated BoNT/A-injected muscles. We next looked at the effects of ouabain on BoNT/A-induced muscle atrophy. Administration of ouabain led to a decrease in the myofibrillar cross-sectional area （CSAs) by 14 post-BoNT/A injection. In addition, repeated administration of ouabain increased mRNA expression levels of ubiquitin ligases, which are markers of muscle atrophy, in BoNT/A-injected muscle. These results suggest that ouabain exacerbates BoNT/A-induced neuromuscular paralysis via a marked progression of BoNT/A-induced muscle atrophy.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Toxicology","subitem_publisher_language":"en"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.2131/jts.35.795","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Japanese Society of Toxicology","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00002808","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03881350","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujikawa, Ryu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muroi, Yoshikage","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Unno, Toshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishii, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"Ouabain.pdf","filesize":[{"value":"5.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Ouabain.pdf","url":"https://obihiro.repo.nii.ac.jp/record/728/files/Ouabain.pdf"},"version_id":"321b7917-0de6-4472-9f86-008e64629860"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ouabain","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Botulinum neurotoxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Muscle atrophy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MAFbx","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MuRF-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ouabain exacerbates botulinum neurotoxin-induced muscle paralysis via progression of muscle atrophy in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ouabain exacerbates botulinum neurotoxin-induced muscle paralysis via progression of muscle atrophy in mice","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-02-28"},"publish_date":"2012-02-28","publish_status":"0","recid":"728","relation_version_is_last":true,"title":["Ouabain exacerbates botulinum neurotoxin-induced muscle paralysis via progression of muscle atrophy in mice"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-28T03:04:32.566369+00:00"}