@article{oai:obihiro.repo.nii.ac.jp:00000645,
 author = {Kawase, Yosuke and Tachibe, Takanori and Hani, Toshio and Tateishi, Hiromi and Jishage, Kou-ichi and Suzuki, Hiroshi},
 issue = {4},
 journal = {Experimental Animals},
 month = {},
 note = {application/pdf, Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is effective for IVF using cryopreserved genetically modified mouse spermatozoa.},
 pages = {415--419},
 title = {Effect of Zona Incision by Piezo-Micromanipulator (ZIP) on In Vitro Fertilization in 21 Transgenic Mice Lines},
 volume = {58},
 year = {2009}
}