@article{oai:obihiro.repo.nii.ac.jp:00000062,
 author = {Miyahara, Kazuro and 宮原, 和郎 and Tose, Shinichi and Sakurai, Haruhisa and Igarashi, Ikuo and 五十嵐, 郁男 and Saito, Atsushi and Hirose, Tsuneo and 広瀬, 恒夫 and Suzuki, Naoyoshi},
 issue = {4},
 journal = {Journal of Veterinary Medical Science},
 month = {},
 note = {application/pdf, In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with anti-macrophage serum prevented induction of cytotoxic activity by TLA. Biologically active IL-2 was not detected in culture supernatants of spleen cells exposed to TLA. These findings suggest that contact between TLA-sensitized non-adherent cells and macrophages is necessary for induction of cytotoxic cells in the presence of TLA. This contact, however, is not necessary for generation of IL-2-induced killer cells.},
 pages = {629--635},
 title = {Role of Adherent Spleen Cells in the Induction of Cytotoxic Activity by Toxoplasma Lysate Antigen},
 volume = {54},
 year = {1992}
}