{"created":"2023-05-15T15:15:05.506545+00:00","id":603,"links":{},"metadata":{"_buckets":{"deposit":"47c05b0c-9fcd-4a3f-a11d-d06f27153e87"},"_deposit":{"created_by":12,"id":"603","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"603"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000603","sets":["5"]},"author_link":["78","1323","1325","1324"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"670","bibliographicPageStart":"664","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"Breeding Science","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"P23k is a monocot-unique protein that is highly expressed in barley. Our previous loss-of-function studies in barley leaves indicated that P23k, localized to tissues where cell wall polysaccharides accumulate, might contribute to secondary wall formation in the leaf. However, the P23k loss-of-function analysis was limited to the leaf, which is a vegetative organ. Considering the involvement of P23k in secondary wall formation, a dramatically altered phenotype is expected in the stem of P23k gene-silenced barley, where marked secondary wall deposition occurs during the reproductive growth stage. To test this hypothesis, barley striped mosaic virus-based virus-induced gene silencing of P23k was performed. Abnormal tiller formation and arrested intercalary elongation were observed in P23k-silenced barley. From these results, we speculated that cell wall architecture was altered by P23k gene silencing. Consistent with this idea, we observed a marked decrease in the amount of cell wall polysaccharides stained with calcofluor and down-regulation of the cellulose synthase-like CslF6 gene involved in (1,3;1,4)-β-D-glucan synthesis. Taken together, these results suggest that P23k is possibly involved in determining secondary wall architecture and contributes to tiller formation and intercalary elongation in barley.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本育種学会","subitem_publisher_language":"ja"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1270/jsbbs.59.664","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本育種学会","subitem_rights_language":"ja"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11353132","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13447610","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Oikawa, Ai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1323","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagai, Kazuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1324","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Kato, Kiyoaki","creatorNameLang":"en"},{"creatorName":"加藤, 清明","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Kato","familyNameLang":"en"},{"familyName":"加藤","familyNameLang":"ja"}],"givenNames":[{"givenName":"Kiyoaki","givenNameLang":"en"},{"givenName":"清明","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"78","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000260369193","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000260369193"},{"nameIdentifier":"60271748","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60271748"},{"nameIdentifier":"read0143695","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0143695"}]},{"creatorNames":[{"creatorName":"Kidou, Shin-ichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1325","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"genesilencing.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"genesilencing.pdf","url":"https://obihiro.repo.nii.ac.jp/record/603/files/genesilencing.pdf"},"version_id":"dd771c61-2e06-4d59-b9f3-032941a6ccb9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hordeum vulgare","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"P23k","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"virus-induced gene silencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"secondary wall formation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"(1,3;1,4)-β-D-glucan","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tiller","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intercalary elongation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Gene silencing of barley P23k involved in secondary wall formation causes abnormal tiller formation and intercalary elongation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene silencing of barley P23k involved in secondary wall formation causes abnormal tiller formation and intercalary elongation","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-11-01"},"publish_date":"2010-11-01","publish_status":"0","recid":"603","relation_version_is_last":true,"title":["Gene silencing of barley P23k involved in secondary wall formation causes abnormal tiller formation and intercalary elongation"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-12-04T02:28:27.692635+00:00"}