{"created":"2023-05-15T15:15:04.407580+00:00","id":577,"links":{},"metadata":{"_buckets":{"deposit":"6b98dd3b-1366-4cff-b726-2af7b8cd162a"},"_deposit":{"created_by":12,"id":"577","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"577"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000577","sets":["5"]},"author_link":[],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Freeze-drying Spermatozoa in Mice","subitem_alternative_title_language":"en"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"27","bibliographicPageStart":"21","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"低温生物工学会誌","bibliographic_titleLang":"ja"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. Unfortunately, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. Since freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential, recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying spermatozoa in an effort to store the male gamete at ambient temperature. It is particularly essential to assure long-term preservation for several decades or centuries. Recent findings indicate that the pressure levels at primary drying of freeze-dried spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa in mice.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"セミナー「低温と乾燥による生物保存技術への挑戦～マミーエンジニアリング」3.","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"低温生物工学会","subitem_publisher_language":"ja"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.20585/cryobolcryotechnol.54.1_21","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"低温生物工学会","subitem_rights_language":"ja"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10448734","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13407902","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 宏志","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"川瀬, 洋介","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"54_KJ00005715547.pdf","filesize":[{"value":"927.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"54_KJ00005715547.pdf","url":"https://obihiro.repo.nii.ac.jp/record/577/files/54_KJ00005715547.pdf"},"version_id":"8ca2f48f-ebe5-4aa3-8811-d0c1d3b5637b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Freese-drying","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Spermatozoa","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"凍結乾燥","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"凍結乾燥によるマウス精子の保存","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"凍結乾燥によるマウス精子の保存","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-20"},"publish_date":"2017-09-20","publish_status":"0","recid":"577","relation_version_is_last":true,"title":["凍結乾燥によるマウス精子の保存"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-28T03:06:19.947099+00:00"}