{"created":"2023-05-15T15:18:29.635531+00:00","id":4889,"links":{},"metadata":{"_buckets":{"deposit":"3f67b635-d0f6-4a8b-93ea-9d6809626a2f"},"_deposit":{"created_by":12,"id":"4889","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"4889"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00004889","sets":["5"]},"author_link":["6818","6819","6792","181","2099"],"control_number":"4889","item_2_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageStart":"509","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"Viruses","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To enhance biosafety and reliability in SARS-CoV-2molecular diagnosis, virus lysis/transport buffers should inactivate the virus and preserve viral RNA under various conditions. Herein, we evaluated the SARS-CoV-2-inactivating activity of guanidine hydrochloride (GuHCl)- and surfactant (hexadecyltrimethylammonium chloride (Hexa-DTMC))-based buffer, Prep Buffer A, (Precision System Science Co., Ltd., Matsudo, Japan) and its efficacy in maintaining the stability of viral RNA at different temperatures using the traditional real-time one-step RT-PCR and geneLEAD VIII sampleto- result platform. Although Prep Buffer A successfully inactivated SARS-CoV-2 in solutions with high and low organic substance loading, there was considerable viral genome degradation at 35 _C compared with that at 4 _C. The individual roles of GuHCl and Hexa-DTMC in virus inactivation and virus genome stability at 35 _C were clarified. Hexa-DTMC alone (0.384%), but not 1.5 M GuHCl alone, exhibited considerable virucidal activity, suggesting that it was essential for potently inactivating SARS-CoV-2 using Prep Buffer A. GuHCl and Hexa-DTMC individually reduced the viral copy numbers to the same degree as Prep Buffer A. Although both components inhibited RNase activity, Hexa-DTMC, but not GuHCl, directly destroyed naked viral RNA. Our findings suggest that samples collected in Prep Buffer A should be stored at 4 _C when RT-PCR will not be performed for several days.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_2_relation_13":{"attribute_name":"item_2_relation_13","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"36851723","subitem_relation_type_select":"PMID"}}]},"item_2_relation_14":{"attribute_name":"item_2_relation_14","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.3390/v15020509","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_9":{"attribute_name":"item_2_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"19994915","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"493","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Komu, James Gitau","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6818","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jamsransuren, Dulamjav","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6819","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuda, Sachiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6792","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Ogawa, Haruko","creatorNameLang":"en"},{"creatorName":"小川, 晴子","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Ogawa","familyNameLang":"en"},{"familyName":"小川","familyNameLang":"ja"}],"givenNames":[{"givenName":"Haruko","givenNameLang":"en"},{"givenName":"晴子","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"181","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10400079","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10400079"},{"nameIdentifier":"obioga","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/obioga"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Takeda, Yohei","creatorNameLang":"en"},{"creatorName":"武田, 洋平","creatorNameLang":"ja"},{"creatorName":"タケダ, ヨウヘイ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Takeda","familyNameLang":"en"},{"familyName":"武田","familyNameLang":"ja"},{"familyName":"タケダ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yohei","givenNameLang":"en"},{"givenName":"洋平","givenNameLang":"ja"},{"givenName":"ヨウヘイ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2099","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30804447","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30804447"},{"nameIdentifier":"ykyyou","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/ykyyou"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-24"}],"displaytype":"detail","filename":"takeda_15_202304.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"fulltext","url":"https://obihiro.repo.nii.ac.jp/record/4889/files/takeda_15_202304.pdf"},"version_id":"a0e0999b-adcf-4503-8782-d6e2230796cc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SARS-CoV-2-inactivation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"virus lysis/transport buffer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA stability","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"saliva","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"guanidine hydrochloride","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficacy Validation of SARS-CoV-2-Inactivation and Viral Genome Stability in Saliva by a Guanidine Hydrochloride and Surfactant-Based Virus Lysis/Transport Buffer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficacy Validation of SARS-CoV-2-Inactivation and Viral Genome Stability in Saliva by a Guanidine Hydrochloride and Surfactant-Based Virus Lysis/Transport Buffer","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-24"},"publish_date":"2023-04-24","publish_status":"0","recid":"4889","relation_version_is_last":true,"title":["Efficacy Validation of SARS-CoV-2-Inactivation and Viral Genome Stability in Saliva by a Guanidine Hydrochloride and Surfactant-Based Virus Lysis/Transport Buffer"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-12-04T02:29:19.494530+00:00"}