@article{oai:obihiro.repo.nii.ac.jp:00000487, author = {Leo, Fiame and Hashida, Shukichi and Kumagai, Daiju and Uchida, Kenji and 内田, 健治 and Motoshima, Hidemasa and Arai, Ikichi and 荒井, 威吉 and Asakuma, Sadaki and 朝隈, 貞樹 and Fukuda, Kenji and 福田, 健二 and Urashima, Tadasu and 浦島, 匡}, issue = {4}, journal = {Journal of Applied Glycoscience}, month = {}, note = {application/pdf, A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6 substituted galactopyranose and O-2,3 disubstituted glucopyranose. The minor saccharide consisted of non-reducing galactopyranose, O-3 substituted glucopyranose, O-6 substituted galactopyranose and O-2,3 disubstituted glucopyranose. The results suggest that these exopolysaccharides are novel saccharides that differ from previously reported products of Lactobacillus fermentum strains. Their presence may contribute to the ropy properties of the medium.}, pages = {223--229}, title = {Studies on a Neutral Exopolysaccharide of Lactobacillus fermentum TDS030603}, volume = {54}, year = {2007} }