{"created":"2023-05-15T15:18:22.310604+00:00","id":4726,"links":{},"metadata":{"_buckets":{"deposit":"a14454d8-e3ac-45cc-a2af-10958e3737cb"},"_deposit":{"created_by":12,"id":"4726","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"4726"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00004726","sets":["12:326"]},"author_link":["5907","176"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"19","bibliographicPageStart":"11","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"The Journal of protozoology research","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Neospora caninum is an apicomplexan parasite that causes abortion in cattle and sheep. Dog\nis the main host of N. caninum and infected one shows neurologic disorders. Accurate\ndiagnosis is important particularly under lacking of treatment and vaccination measures.\nHerein, we targeted the evaluation of diagnostic ability of recombinant protein\nNcGRA1(rNcGRA1) for detection of specific antibody (IgG) against N. caninum in mice and\ndogs. The rNcGRA1 was expressed using the E. coli expression system. Diagnostic ability\nof rNcGRA1 was tested against experimental mouse and dog sera, as well as field dog sera.\nSimultaneously, same serum samples were tested against rNcSAG1 and Neospora lysate\nantigen (NLA)-based iELISA. In the N. caninum experimentally infected mice, specific IgG\nantibodies to NcGRA1 were detectable from 14 days post infection (dpi), and increased at\n28 dpi with no detectable antibody levels in the negative control or Toxoplasma gondii\ninfected-mouse sera. In experimentally infected dog sera, the performance of rNcGRA1 was\nproficient in demonstrating IgG antibody at 28 dpi. In case of field dog sera (N=18),\nseropositive rate was 44.4% and 22.2% for rNcGRA1 and rNcSAG1-based iELISAs,\nrespectively. These results show that the rNcGRA1 is a novel useful diagnostic antigen for\nserological detection of N. caninum in dogs.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24556/00004708","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Research Center for Protozoan Diseases","subitem_publisher_language":"en"}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"National Research Center for Protozoan Diseases","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824856","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09174427","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Abdelbaky, Hanan H.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Nishikawa, Yoshifumi","creatorNameLang":"en"},{"creatorName":"西川, 義文","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-24"}],"displaytype":"detail","filename":"JPR_30_11-19_2020.pdf","filesize":[{"value":"462.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JPR_30_11-19_2020","url":"https://obihiro.repo.nii.ac.jp/record/4726/files/JPR_30_11-19_2020.pdf"},"version_id":"27675c15-b1c4-41c3-b58e-4f00e3af9f5c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Neospora caninum; Neosporosis; NcGRA1; ELISA; Dog","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Usefulness of Neospora caninum dense granule 1 based-iELISA for the detection of canine neosporosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Usefulness of Neospora caninum dense granule 1 based-iELISA for the detection of canine neosporosis","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["326"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-02-24"},"publish_date":"2021-02-24","publish_status":"0","recid":"4726","relation_version_is_last":true,"title":["Usefulness of Neospora caninum dense granule 1 based-iELISA for the detection of canine neosporosis"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-20T07:45:19.488293+00:00"}