@article{oai:obihiro.repo.nii.ac.jp:00004469,
 author = {Hayashi, Masahiro and Natori, Tatsuya and Kubota-Hayashi, Sayoko and Miyata, Machiko and Ohkusu, Kiyofumi and Kawamoto, Keiko and 川本, 恵子 and Kurazono, Hisao and Makino, Souichi and Ezaki, Takayuki},
 journal = {BioMed Research International},
 month = {},
 note = {application/pdf, A quick foodborne pathogen screening method after six-hour enrichment culture with a broad-range food pathogen enrichment broth is described. Pathogenic factors of Salmonella enterica, Shigella spp., enteroinvasive Escherichia coli, and enterohemorrhagic E. coli are amplified with a cocktail primer and rapid polymerase chain reaction (PCR), which finishes amplification in 30 min. The PCR amplicon was differentiated with a dipstick DNA chromatography assay in 5-10 min. Starting from a four- to six-hour enrichment culture, this assay was finished within 45 min. Detection sensitivity of this protocol was less than 2.5 CFU/25 g for S. enterica and 3.3 CFU/25 g for enterohemorrhagic E. coli in spiked ground meat experiments. © 2013 Masahiro Hayashi et al.},
 title = {A new protocol to detect multiple foodborne pathogens with PCR dipstick DNA chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth},
 volume = {2013},
 year = {2013}
}