@article{oai:obihiro.repo.nii.ac.jp:00004450,
 author = {Shirasuna, Koumei and Jiemtaweeboon, Sineenard and Raddatz, Sybille and Nitta, Akane and Schuberth, Hans-Joachim and Bollwein, Heinrich and Shimizu, Takashi and 清水, 隆 and Miyamoto, Akio and 宮本, 明夫},
 issue = {1},
 journal = {PLoS ONE},
 month = {},
 note = {application/pdf, Prostaglandin F 2α (PGF 2α) induces luteolysis within a few days in cows, and immune cells increase in number in the regressing corpus luteum (CL), implying that luteolysis is an inflammatory-like immune response. We investigated the rapid change in polymorphonuclear neutrophil (PMN) numbers in response to PGF 2α administration as the first cells recruited to inflammatory sites, together with mRNA of interleukin-8 (IL-8: neutrophil chemoattractant) and P-selectin (leukocyte adhesion molecule) in the bovine CL. CLs were collected by ovariectomy at various times after PGF 2α injection. The number of PMNs was increased at 5 min after PGF 2α administration, whereas IL-8 and P-selectin mRNA increased at 30 min and 2 h, respectively. PGF 2α directly stimulated P-selectin protein expression at 5-30 min in luteal endothelial cells (LECs). Moreover, PGF 2α enhanced PMN adhesion to LECs, and this enhancement by PGF 2α was inhibited by anti-P-selectin antibody, suggesting that P-selectin expression by PGF 2α is crucial in PMN migration. In conclusion, PGF 2α rapidly induces the accumulation of PMNs into the bovine CL at 5 min and enhances PMN adhesion via P-selectin expression in LECs. It is suggested that luteolytic cascade by PGF 2α may involve an acute inflammatory-like response due to rapidly infiltrated PMNs.},
 title = {Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F 2α-induced luteolysis in the cow},
 volume = {7},
 year = {2012}
}