{"created":"2023-05-15T15:18:09.260320+00:00","id":4448,"links":{},"metadata":{"_buckets":{"deposit":"8113041e-fdf3-405c-9607-d1767777f351"},"_deposit":{"created_by":12,"id":"4448","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"4448"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00004448","sets":["5"]},"author_link":["2370","6326","88","6327","151","6324","6325"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"126","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"BMC Veterinary Research","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Plasma concentration of retinol is an accepted indicator to assess the vitamin A (retinol) status in cattle. However, the determination of vitamin A requires a time consuming multi-step procedure, which needs specific equipment to perform extraction, centrifugation or saponification prior to high-performance liquid chromatography (HPLC). Methods: The concentrations of retinol in whole blood (n = 10), plasma (n = 132) and serum (n = 61) were measured by a new rapid cow-side test (iCheck™ FLUORO) and compared with those by HPLC in two independent laboratories in Germany (DE) and Japan (JP). Results: Retinol concentrations in plasma ranged from 0.033 to 0.532 mg/L, and in serum from 0.043 to 0.360 mg/L (HPLC method). No significant differences in retinol levels were observed between the new rapid cow-side test and HPLC performed in different laboratories (HPLC vs. iCheck™ FLUORO: 0.320 ± 0.047 mg/L vs. 0.333 ± 0.044 mg/L, and 0.240 ± 0.096 mg/L vs. 0.241 ± 0.069 mg/L, lab DE and lab JP, respectively). A similar comparability was observed when whole blood was used (HPLC vs. iCheck™ FLUORO: 0.353 ± 0.084 mg/L vs. 0.341 ± 0.064 mg/L). Results showed a good agreement between both methods based on correlation coefficients of r2 = 0.87 (P &lt; 0.001) and Bland-Altman blots revealed no significant bias for all comparison. Conclusions: With the new rapid cow-side test (iCheck™ FLUORO) retinol concentrations in cattle can be reliably assessed within a few minutes and directly in the barn using even whole blood without the necessity of prior centrifugation. The ease of the application of the new rapid cow-side test and its portability can improve the diagnostic of vitamin A status and will help to control vitamin A supplementation in specific vitamin A feeding regimes such as used to optimize health status in calves or meat marbling in Japanese Black cattle. ","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central","subitem_publisher_language":"en"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1186/s12917-017-1042-3","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.1/>","subitem_rights_language":"en"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"17466148","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"645","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Raila, Jens","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Kawashima, Chiho","creatorNameLang":"en"},{"creatorName":"川島, 千帆","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Sauerwein, Helga","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hülsmann, Nadine","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Knorr, Christoph","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Miyamoto, Akio","creatorNameLang":"en"},{"creatorName":"宮本, 明夫","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Schweigert, Florian J.","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-24"}],"displaytype":"detail","filename":"s12917-017-1042-3.pdf","filesize":[{"value":"593.7 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"fulltest","url":"https://obihiro.repo.nii.ac.jp/record/4448/files/s12917-017-1042-3.pdf"},"version_id":"1c31fb53-db8a-49de-9e84-9e614a5c3ff5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Biomarker","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Blood","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cattle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cow-side assay","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Method comparison","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Vitamin A","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Validation of blood vitamin A concentrations in cattle: Comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Validation of blood vitamin A concentrations in cattle: Comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC)","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-10-24"},"publish_date":"2019-10-24","publish_status":"0","recid":"4448","relation_version_is_last":true,"title":["Validation of blood vitamin A concentrations in cattle: Comparison of a new cow-side test (iCheck™ FLUORO) with high-performance liquid chromatography (HPLC)"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-04-30T07:01:16.818888+00:00"}