{"created":"2023-05-15T15:18:09.008064+00:00","id":4444,"links":{},"metadata":{"_buckets":{"deposit":"b9cc579a-50be-416a-961a-2b46eb1447fb"},"_deposit":{"created_by":12,"id":"4444","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"4444"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00004444","sets":["5"]},"author_link":["6314","6019","6316","6315","6317","6313","176"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-02","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"101961","bibliographicVolumeNumber":"74","bibliographic_titles":[{"bibliographic_title":"Parasitology International","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Natural resources are recognized as important sources of potential drugs for treating various infections, and microorganisms are a rich natural source of diverse compounds. Among the world's microorganisms, actinomycetes, which are abundant in soil and marine, are the well-known producers of a wide range of bioactive secondary metabolites and antibiotics. In the present study, four actinomycetes (samples N25, N6, N18, and N12) were isolated from soil samples in Mongolia. Phylogenetic analysis of these isolates revealed that they share the highest similarity with Streptomyces canus (N25), S. cirratus (N6), S. bacillaris (N18) and S. peucetius (N12), based on 16S rRNA gene sequencing. Crude extracts were obtained from them using ethyl acetate, and the crude fractions were separated by thin layer chromatography. The fractions were then evaluated for their cytotoxicities and their anti-Toxoplasma and antimalarial activities in vitro. The S. canus (N25) crude extract was selected for further chemical characterization based on its antiprotozoal activities. Using liquid chromatography-high resolution mass spectrometry, phenazine-1-carboxylic acid (PCA) was detected and identified in the active fractions of the metabolites from strain N25. We next confirmed that commercially available PCA possesses antiprotozoal activity against T. gondii (IC50: 55.5 μg/ml) and Plasmodium falciparum (IC50: 6.4 μg/ml) in vitro. The results of this study reveal that soil actinomycetes are potential sources of antiprotozoal compounds, and that PCA merits further investigation as an anti-protozoal agent.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Ireland","subitem_publisher_language":"en"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.parint.2019.101961","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.3/>","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11112001","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13835769","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Pagmadulam, Baldorj","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tserendulam, Dugarsuren","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rentsenkhand, Tserennadmid","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sawa, Ryuichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nihei, Coh-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Nishikawa, Yoshifumi","creatorNameLang":"en"},{"creatorName":"西川, 義文","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-01"}],"displaytype":"detail","filename":"OAK_PARINT_2019_119_Revision 1_V0.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"fulltext","url":"https://obihiro.repo.nii.ac.jp/record/4444/files/OAK_PARINT_2019_119_Revision 1_V0.pdf"},"version_id":"db59a231-0c3a-462b-b336-d5a2ca19568a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Actinomycetes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mongolia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phenazine-1-carboxylic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmodium falciparum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptomyces sp.","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Toxoplasma gondii","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation and characterization of antiprotozoal compound-producing Streptomyces species from Mongolian soils","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation and characterization of antiprotozoal compound-producing Streptomyces species from Mongolian soils","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-02-01"},"publish_date":"2021-02-01","publish_status":"0","recid":"4444","relation_version_is_last":true,"title":["Isolation and characterization of antiprotozoal compound-producing Streptomyces species from Mongolian soils"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-28T03:13:26.595489+00:00"}